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PMID: 4672667 Published · ppublish English Journal Article

6-phosphogluconate dehydrogenase and the assay of uridine diphosphate glucose pyrophosphorylase in the cellular slime mould Dictyostelium discoideum.

The Biochemical journal ·Vol. 126 ·No. 3 ·1972-02-00 ·Pages 593-600

Edmundson TD, Ashworth JM

Abstract

1. 6-Phosphogluconate dehydrogenase activity is present in all morphogenetic stages during cell differentiation in the cellular slime mould. 2. The different ratios of 6-phosphogluconate dehydrogenase/UDP-glucose pyrophosphorylase observed during this process can render spectrophotometric assays of UDP-glucose pyrophosphorylase inaccurate. 3. The disputed occurrence of increases in specific activity of UDP-glucose pyrophosphorylase during cell differentiation in the cellular slime mould is discussed in the light of these observations.

MeSH Terms
Cell Differentiation Cell-Free System Myxomycetes/enzymology Nucleotidyltransferases/analysis Phosphogluconate Dehydrogenase/analysis Spectrophotometry Spores, Fungal/enzymology
Chemicals
Phosphogluconate Dehydrogenase Nucleotidyltransferases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Edmundson T D
Ashworth J M
References (22)
22 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1260593
Published
1972-02-00
Pages
593-600
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1178416
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1260593 PMC Full Text View journal details
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