Initiation of mammalian protein synthesis: the importance of ribosome and initiation factor quality for the efficiency of in vitro systems.

Animals Brain/metabolism Centrifugation, Density Gradient Globins/biosynthesis Guinea Pigs In Vitro Techniques Liver/metabolism Magnesium/pharmacology Methods Mice Osmolar Concentration Peptide Chain Initiation, Translational Peptide Initiation Factors/isolation & purification Peptide Termination Factors Polyribosomes Potassium/pharmacology Protein Biosynthesis RNA, Messenger/isolation & purification Rabbits Reticulocytes Ribosomes

Peptide Initiation Factors Peptide Termination Factors RNA, Messenger Globins Magnesium Potassium