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PMID: 46910 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Relation of GIX antigen of thymocytes to envelope glycoprotein of murine leukemia virus.

The Journal of experimental medicine ·Vol. 141 ·No. 1 ·1975-01-01 ·Pages 188-97

Obata Y, Ikeda H, Stockert E, Boyse EA

Abstract

Expression of Gix surface antigen on thymocytes is an inherited mendelian train of certain strains of mice. We report here the following new findings: (a) Gix antigen was found free in the serum of Gix+ mouse strains. (b) Expression vs. nonexpression of Gix antigen was invariably correlated with presence or absence of the group-specific antigen of Murine leukemia virus (MuLV) gp69/71 in the serum of mice of inbred and segregating populations. (c) Gix antigen could be removed from normal Gix+ mouse serum by precipitation with antiserum to MuLV gp 69/71. (d) Anti-gp69/71 serum was weakly cytotoxic for Gix+ thymocytes, and partially blocked the cytotoxic activity of Gix antibody for Gix+ thymocytes. (e) Purified AKR virus absorbed Gix activity, and disruption of the virions did not increase their absorbing capacity. These serological data indicate that Gix antigen is a constituent of gp69/71, the glycoprotein which is the major component of the MuLV envelope. On present evidence, Gix antigen is represented in intact virions and is probably accessible to Gix antibody.

MeSH Terms
Absorption Animals Antibodies Antibodies, Viral/analysis Antigens, Viral/analysis Cell Membrane/immunology Complement System Proteins Cytotoxicity Tests, Immunologic Epitopes Fluorescent Antibody Technique Genotype Glycoproteins/immunology Immune Sera Isoantigens/analysis Leukemia Virus, Murine/immunology Mice Mice, Inbred Strains Molecular Biology Phenotype T-Lymphocytes/immunology Viral Proteins/analysis
Chemicals
Antibodies Antibodies, Viral Antigens, Viral Epitopes Glycoproteins Immune Sera Isoantigens Viral Proteins Complement System Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Obata Y
Ikeda H
Stockert E
Boyse E A
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18 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.141.1.188
Published
1975-01-01
Pages
188-97
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2190505
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.141.1.188 PMC Full Text View journal details
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