Home LiteratureArticle Details
PMID: 4691393 Published · ppublish English Journal Article

The role of the Golgi complex in sulfate metabolism.

The Journal of cell biology ·Vol. 57 ·No. 1 ·1973-04-00 ·Pages 175-89

Young RW

Abstract

This investigation was designed to determine if sulfate metabolism is the function of a particular cell organelle, or whether the site of sulfation varies, depending upon the type of cell and the class of sulfated compound. Rats and mice were injected intravenously with inorganic sulfate labeled with (35)S (H(2) (35)SO(4)), and were then killed by vascular perfusion of fixative 5-30 min later. Several tissues were prepared for electron microscope autoradiography. 14 different types of specialized cells which incorporated the labeled sulfate were analyzed. In every case, the sulfate was initially detected in the smooth membranes and vesicles of the Golgi complex. Available evidence indicates that these cells were engaged in the synthesis of several different sulfated compounds, including mucopolysaccharides, glycoproteins, lipids, and steroids. These results lead to the generalization that the enzymes required for the transfer of inorganic sulfate to a variety of acceptor molecules are located in the Golgi complex.

MeSH Terms
Animals Autoradiography Basophils Blood Vessels/cytology Bone Marrow Cells Cornea/cytology Endothelium/cytology Eosinophils Female Fibroblasts Golgi Apparatus/analysis,metabolism Histocytochemistry Mast Cells Megakaryocytes Methods Mice Microscopy, Electron Neuroglia Neutrophils Ovary/cytology Schwann Cells Sulfates/isolation & purification,metabolism Sulfur Isotopes
Chemicals
Sulfates Sulfur Isotopes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Young R W
References (40)
40 references, click to expand
  1. Cytochemical properties of the blood basophilic granulocyte.
    Ann N Y Acad Sci. 1963 Feb 26;103:376-93 PMID: 14010874
  2. Sulfated glycopeptides and glycosaminoglycan peptides isolated from intestinal mucosae of rabbit.
    Biochim Biophys Acta. 1969 Oct 7;192(1):37-48 PMID: 4242312
  3. ACID MUCOPOLYSACCHARIDES IN HUMAN LEUCOCYTES.
    Clin Chim Acta. 1963 Jul;8:505-12 PMID: 14063962
  4. ELECTRON MICROSCOPE STUDY ON PRIMARY LIQUOR FOLLICULI SECRETION IN THE MOUSE OVARY.
    J Ultrastruct Res. 1963 Dec;52:445-58 PMID: 14088994
  5. Sulfated glycopeptides from rat brain glycoproteins.
    Biochemistry. 1970 Oct 27;9(22):4389-96 PMID: 4248631
  6. Autoradiographic visualization of S35 incorporation and turnover by the mucous glands of the gastrointestinal tract and other soft tissues of rat and hamster.
    Anat Rec. 1954 Apr;118(4):755-71 PMID: 13158875
  7. SULFATED MUCOPOLYSACCHARIDE AND BASIC PROTEIN IN CERTAIN GRANULES OF RABBIT LEUKOCYTES.
    Lab Invest. 1964 Jan;13:1-15 PMID: 14104473
  8. Androgen sulphate formation in male and female mice.
    Biochem J. 1969 Nov;115(3):489-93 PMID: 4242857
  9. Subcellular sites for synthesis of glycosaminoglycans (mucopolysaccharides) of rabbit bone marrow cells.
    Exp Cell Res. 1972 Jan;70(1):173-84 PMID: 4257607
  10. [Electron microscopic picture of the ovary of juvenile rats and rabbits after stimulation with pregnant mare serum (PMS) and human chorionic gonadotropin (HCG). I. Theca and stroma (interstitial gland)].
    Z Zellforsch Mikrosk Anat. 1969;94(4):605-23 PMID: 5809192
  11. A histochemical and radioautographic study of the participation of fibroblasts in the production of mucopolysaccharides in connective tissue.
    J Histochem Cytochem. 1961 May;9:278-91 PMID: 14469152
  12. Studies on the mechanism of ovulation: isolation and analysis of acid mucopolysaccharides in bovine follicular fluid.
    Acta Endocrinol (Copenh). 1958 Mar;27(3):356-68 PMID: 13507992
  13. The acid mucopolysaccharides of connective tissue.
    Biochim Biophys Acta. 1956 Sep;21(3):506-18 PMID: 13363957
  14. The nature of sulphation of uronic acid-containing glycosaminoglycans catalysed by brain sulphotransferase.
    J Neurochem. 1970 Feb;17(2):189-200 PMID: 4250217
  15. The sulfolipids.
    J Lipid Res. 1961 Apr;2:103-9 PMID: 13706453
  16. The intracellular transport of glycosaminoglycans (mucopolysaccharides) in human leukocytes.
    Exp Cell Res. 1969 Mar;54(3):318-25 PMID: 5252201
  17. Synthesis of sulphatide-containing lipoproteins in rat brain.
    J Neurochem. 1969 Jul;16(7):1049-57 PMID: 5796862
  18. ISOLATION AND PRELIMINARY CHARACTERIZATION OF RAT MAST CELL GRANULES.
    Lab Invest. 1964 Nov;13:1331-44 PMID: 14226497
  19. Histochemical demonstration of sulfated mucosubstances and cationic proteins in human granulocytes and platelets.
    J Histochem Cytochem. 1969 Oct;17(10):668-74 PMID: 4194355
  20. Origin of granules in polymorphonuclear leukocytes. Two types derived from opposite faces of the Golgi complex in developing granulocytes.
    J Cell Biol. 1966 Feb;28(2):277-301 PMID: 5914694
  21. Segregation and packaging of granule enzymes in eosinophilic leukocytes.
    J Cell Biol. 1970 Apr;45(1):54-73 PMID: 5459000
  22. The structure of bone marrow. Functional interrelationships of vascular and hematopoietic compartments in experimental hemolytic anemia: an electron microscopic study.
    J Morphol. 1965 Nov;117(3):467-537 PMID: 5883927
  23. ON THE SITE OF SULFATION IN THE CHONDROCYTE.
    J Cell Biol. 1964 Jun;21:353-66 PMID: 14189910
  24. The uptake of radioactive sulphate by cells, fibres and ground-substance of mature and developing connective tissue in the adult mouse.
    J Anat. 1956 Oct;90(4):478-85 PMID: 13366859
  25. Isolation and characterization of glycosaminoglycans from human leukocytes and platelets.
    Biochim Biophys Acta. 1967 Jul 25;141(2):348-57 PMID: 6057662
  26. Ultrastructural localization of acid mucosubstance and antimonate-precipitable cation in human and rabbit platelets and megakaryocytes.
    J Histochem Cytochem. 1969 Dec;17(12):781-92 PMID: 4189436
  27. Isolation and characterization of glycosaminoglycans in peripheral nerve and spinal cord of monkey.
    J Neurochem. 1969 Nov;16(11):1529-32 PMID: 4245162
  28. Utilization of sulfate ion by eosinophile myelocytes in the rat.
    Exp Cell Res. 1956 Aug;11(1):225-8 PMID: 13356848
  29. An autoradiographic study on the uptake of S35-labelled sodium sulphate in the mast cells.
    Acta Haematol. 1953 May;9(5):273-6 PMID: 13064997
  30. ON THE SITE OF SULFATION IN COLONIC GOBLET CELLS.
    J Cell Biol. 1964 Jun;21:339-51 PMID: 14193835
  31. Autoradiographic analysis of sulfate metabolism in the cerebellum of the mouse.
    Brain Res. 1971 Jul 23;30(2):295-309 PMID: 4255609
  32. Ultrastructural localization of dialyzed iron-reactive mucosubstance in rabbit heterophils, basophils, and eosinophils.
    J Cell Biol. 1971 Feb;48(2):368-86 PMID: 4101523
  33. Ultrastructure of human mast-cell granules.
    J Ultrastruct Res. 1968 Apr;23(1):153-65 PMID: 5670837
  34. Sulfate metabolism in pancreatic acinar cells.
    J Cell Biol. 1971 Aug;50(2):469-83 PMID: 5112646
  35. Autoradiographic observations on the uptake of S35 in the genital organs of the female rat and rabbit after injection of labeled sodium sulfate.
    Acta Endocrinol (Copenh). 1952 May;10(1):89-96 PMID: 14943405
  36. [Problems of electron microscopic radioautography].
    Naturwissenschaften. 1966 Jun;53(11):268-71 PMID: 5987029
  37. A time-picture relation study with autoradiography on the uptake of labelled sulphate in the graafian follicles of the rabbit.
    Acta radiol. 1953 Feb;39(2):137-40 PMID: 13040065
  38. Microscopic distribution of radioisotopes in tissues.
    Lab Invest. 1953 Jul-Aug;2(4):264-79 PMID: 13070540
  39. Utilization of sulphate ion by fibroblasts in the quartz focus.
    Nature. 1955 Mar 5;175(4453):435-6 PMID: 14356202
  40. Histochemistry and characterization of hyaluronic acid in axons of peripheral nerve.
    J Neurochem. 1956 Dec;1(2):119-25 PMID: 13398826
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.57.1.175
Published
1973-04-00
Pages
175-89
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108953
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.57.1.175 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2