Production of specific fragments of phi X174 replicative form DNA by a restriction enzyme from Haemophilus parainfluenzae, endonuclease HP.

Journal of virology ·Vol. 11 ·No. 4 ·1973-04-00 ·Pages 596-9

Johnson PH, Lee AS, Sinsheimer RL

Abstract

A restriction endonuclease from Haemophilus parainfluenzae degrades phiX174 replicative form DNA into eight specific fragments, ranging from 1,700 to 150 base pairs and terminated specifically by deoxycytidylic acid.

MeSH Terms

Ammonium Sulfate Cell-Free System Centrifugation, Density Gradient Chemical Precipitation Chromatography, DEAE-Cellulose Chromatography, Gel Coliphages/metabolism DNA Replication/drug effects DNA Viruses/metabolism DNA, Viral/analysis,biosynthesis,metabolism Deoxyribonucleases/isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Endonucleases/pharmacology Haemophilus/enzymology Nucleotides/analysis Tritium

Chemicals

DNA, Viral Nucleotides Tritium Deoxyribonucleases Endonucleases Ammonium Sulfate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Johnson P H

Lee A S

Sinsheimer R L

References (7)

7 references, click to expand

Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli.
Proc Natl Acad Sci U S A. 1965 Jul;54(1):158-65 PMID: 5323016
A restriction enzyme from Hemophilus influenzae. I. Purification and general properties.
J Mol Biol. 1970 Jul 28;51(2):379-91 PMID: 5312500
A restriction enzyme from Hemophilus influenzae. II.
J Mol Biol. 1970 Jul 28;51(2):393-409 PMID: 5312501
Specific cleavage of simian virus 40 DNA by restriction endonuclease of Hemophilus influenzae.
Proc Natl Acad Sci U S A. 1971 Dec;68(12):2913-7 PMID: 4332003
Action of haemophilus endodeoxyribonuclease on biologically active deoxyribonucleic acid.
J Bacteriol. 1972 Mar;109(3):987-92 PMID: 4334766
Specific endonuclease R fragments of bacteriophage phiX174 deoxyribonucleic acid.
J Virol. 1972 Apr;9(4):574-82 PMID: 4553678
Specific fragments of phi X174 deoxyribonucleic acid produced by a restriction enzyme from Haemophilus aegyptius, endonuclease Z.
J Virol. 1972 Jul;10(1):42-50 PMID: 4537735

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.11.4.596-599.1973

Published

1973-04-00

Pages

596-9

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC355142

Subset

External Links

PubMed source DOI: 10.1128/JVI.11.4.596-599.1973 PMC Full Text View journal details

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Production of specific fragments of phi X174 replicative form DNA by a restriction enzyme from Haemophilus parainfluenzae, endonuclease HP.

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