Control of ornithine decarboxylase activity in stimulated human lymphocytes by putrescine and spermidine.

The Biochemical journal ·Vol. 132 ·No. 4 ·1973-04-00 ·Pages 791-6

Kay JE, Lindsay VJ

Abstract

Ornithine decarboxylase activity in extracts of phytohaemagglutinin-stimulated human lymphocytes is rapidly and extensively inhibited by additions of micromolar concentrations of putrescine or spermidine to the culture medium. This inhibition is not due to feedback inhibition of the enzyme by putrescine, spermidine or their metabolites. Inhibition is dependent on the continuation of protein synthesis, but does not require RNA synthesis. The effect of putrescine is abolished when its conversion into spermidine by the cells is prevented.

MeSH Terms

Blood Proteins/biosynthesis Carbon Isotopes Carboxy-Lyases/blood Cells, Cultured Cycloheximide/pharmacology DNA/biosynthesis Humans Lectins/pharmacology Lymphocytes/drug effects,enzymology Ornithine Phenylalanine/metabolism Putrescine/pharmacology RNA/biosynthesis S-Adenosylmethionine Spermidine/pharmacology Thymidine/metabolism Time Factors Tritium Uridine/metabolism

Chemicals

Blood Proteins Carbon Isotopes Lectins Tritium Phenylalanine RNA S-Adenosylmethionine DNA Cycloheximide Ornithine Carboxy-Lyases Spermidine Putrescine Thymidine Uridine

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kay J E

Lindsay V J

References (11)

11 references, click to expand

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Putrescine and spermidine biosynthesis in the development of normal and anucleolate mutants of Xenopus laevis.
Proc Natl Acad Sci U S A. 1971 Mar;68(3):523-7 PMID: 5276754
Amine synthesis in regenerating rat liver: extremely rapid turnover of ornithine decarboxylase.
Mol Pharmacol. 1969 May;5(3):253-62 PMID: 5783961
Effect of cycloheximide on protein and ribonucleic acid synthesis in cultured human lymphocytes.
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FEBS Lett. 1972 Mar 15;21(2):123-126 PMID: 11946491
Amino acid metabolism in mammalian cell cultures.
Science. 1959 Aug 21;130(3373):432-7 PMID: 13675766
Ribonucleic acid metabolism in lymphocytes stimulated by phytohemagglutinin. II. Rapidly synthesized ribonucleic acid and te production of ribosomal ribonucleic acid.
J Biol Chem. 1968 Jan 10;243(1):34-43 PMID: 5635946
Studies on ornithine decarboxylase from the liver of thioacetamide-treated rats. Purification and some properties.
Biochim Biophys Acta. 1972 Sep 19;284(1):285-97 PMID: 5073764
Biosynthesis of putrescine in the prostate gland of the rat.
Biochem J. 1968 Jul;108(4):533-9 PMID: 5667265
Stimulation of the decarboxylation of S-adenosylmethionine by putrescine in mammalian tissues.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1320791

Published

1973-04-00

Pages

791-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177653

Subset

External Links

PubMed source DOI: 10.1042/bj1320791 PMC Full Text View journal details

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Control of ornithine decarboxylase activity in stimulated human lymphocytes by putrescine and spermidine.

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