Phosphorylation of the "37000 component" of the troponin complex (troponin-t).

The Biochemical journal ·Vol. 131 ·No. 2 ·1973-02-00 ·Pages 425-8

Perry SV, Cole HA

Abstract

Most of the phosphorus present in the troponin complex, which on average contains 1 mol of P/80000g, is associated with the ;37000 component' (0.6mol of P/mol). The inhibitory protein and particularly the ;37000 component', but not the calcium-binding protein, were phosphorylated when incubated with phosphorylase b kinase and [gamma-(32)P]ATP.

MeSH Terms

Animals Binding Sites Calcium Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel Kinetics Muscle Proteins/analysis,metabolism Phosphates/analysis Phosphorus Isotopes Phosphorylase Kinase/metabolism Protein Binding Rabbits Time Factors

Chemicals

Muscle Proteins Phosphates Phosphorus Isotopes Phosphorylase Kinase Calcium

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Perry S V

Cole H A

References (12)

12 references, click to expand

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PURIFICATION AND PROPERTIES OF RABBIT SKELETAL MUSCLE PHOSPHORYLASE B KINASE.
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Troponin and its components.
J Biochem. 1971 Feb;69(2):441-5 PMID: 5550981
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
An adenosine 3',5'-monophosphate-dependant protein kinase from rabbit skeletal muscle.
J Biol Chem. 1968 Jul 10;243(13):3763-5 PMID: 4298072
The regulatory proteins of the myofibril. Separation and biological activity of the components of inhibitory-factor preparations.
Biochem J. 1972 Mar;127(1):215-28 PMID: 4262960
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.
J Biol Chem. 1971 Aug 10;246(15):4866-71 PMID: 4254541
Separation and characterization of the constituents of troponin.
FEBS Lett. 1971 Jan 12;12(3):148-152 PMID: 11945563
Separation and characterization of the inhibitory factor of the troponin system.
Biochem Biophys Res Commun. 1971 Jul 2;44(1):237-44 PMID: 5116514
Phosphorylation of the inhibitor component of troponin by phosphorylase kinase.
J Biol Chem. 1972 Aug 25;247(16):5272-4 PMID: 4262569
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Phosphorylation of rabbit muscle troponin and actin by a 3', 5'-c-AMP-dependent protein kinase.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1310425

Published

1973-02-00

Pages

425-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177485

Subset

External Links

PubMed source DOI: 10.1042/bj1310425 PMC Full Text View journal details

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Phosphorylation of the "37000 component" of the troponin complex (troponin-t).

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