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PMID: 4734321 Published · ppublish English Journal Article

Characterization of nucleic acid of pichinde virus.

Journal of virology ·Vol. 11 ·No. 1 ·1973-01-00 ·Pages 61-8

Carter MF, Biswal N, Rawls WE

Abstract

The nucleic acid of Pichinde virus was found to be single-stranded ribonucleic acid (RNA) as determined by sensitivity to ribonuclease, by alkaline degradation, by buoyant density in cesium sulfate, and by analysis of the base composition. The RNA of the virion could be separated into five components which had sedimentation coefficients corresponding to 31S, 28S, 22S, 18S and 4 to 6S. The 28S, 18S, and possibly the 4 to 6S RNAs appear to be derived from host cell components incorporated into the virion, whereas the 31S and 22S components appear to represent the genome of the virus.

MeSH Terms
Animals Carbon Isotopes Cell Line Centrifugation, Density Gradient Cricetinae Cytosine/analysis Dactinomycin/pharmacology Electrophoresis, Paper Electrophoresis, Polyacrylamide Gel Guanosine/analysis HeLa Cells Humans Kidney Molecular Weight Phosphorus RNA Viruses/analysis,growth & development,isolation & purification RNA, Ribosomal/analysis RNA, Viral/analysis,isolation & purification Ribonucleases Tritium
Chemicals
Carbon Isotopes RNA, Ribosomal RNA, Viral Tritium Guanosine Dactinomycin Phosphorus Cytosine Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Carter M F
Biswal N
Rawls W E
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25 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.1.61-68.1973
Published
1973-01-00
Pages
61-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355061
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.1.61-68.1973 PMC Full Text View journal details
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