Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans.

Infection and immunity ·Vol. 7 ·No. 2 ·1973-02-00 ·Pages 237-41

Evans RT, Genco RJ

Abstract

Using a recently developed assay for glucosyltransferase activity based on (14)C-glucose incorporation into an alcohol-insoluble polysaccharide, we were able to study inhibition by antibody of this enzyme activity. Rabbit antibody was relatively specific for the strain of Streptoccus mutans from which the enzyme was obtained. Absorption studies showed that neither removal of antibodies directed to dextran nor absorption with intact bacteria offset the enzyme-inhibitory capacity of these sera, whereas absorption with partially purified enzyme did result in removal of the inhibitory capacity.

MeSH Terms

Absorption Animals Carbon Isotopes Dental Plaque/microbiology Enzyme Repression Glucose/metabolism Glucosyltransferases/antagonists & inhibitors,metabolism Immune Sera/pharmacology Polysaccharides/metabolism Rabbits/immunology Streptococcus/enzymology,immunology Tritium

Chemicals

Carbon Isotopes Immune Sera Polysaccharides Tritium Glucosyltransferases Glucose

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Evans R T

Genco R J

References (12)

12 references, click to expand

The extracellular polysaccharide produced from sucrose by a cariogenic streptococcus.
Arch Oral Biol. 1966 Oct;11(10):1039-42 PMID: 5226783
Synthesis of extracellular dextran by cariogenic bacteria and its presence in human dental plaque.
Arch Oral Biol. 1967 Jan;12(1):11-23 PMID: 4960207
Biochemical and morphological aspects of extracellular polysaccharides produced by cariogenic streptococci.
Helv Odontol Acta. 1967 Oct;11(2):131-52 PMID: 6056776
An in vitro method for assessing the plaque forming ability of oral bacteria.
Arch Oral Biol. 1967 Dec;12(12):1653-6 PMID: 5237345
A dextransucrase activity from Streptococcus FA-1.
Arch Oral Biol. 1967 Dec;12(12):1659-60 PMID: 5237347
Inhibition of streptococcal dextransucrase by sera of rabbits infected with Streptococcus sanguis.
Arch Oral Biol. 1968 Jul;13(7):849-52 PMID: 5244310
Synthesis of insoluble dextran and its significance in the formation of gelatinous deposits by plaque-forming streptococci.
Arch Oral Biol. 1968 Oct;13(10):1249-62 PMID: 5250250
Purification and properties of dextransucrase from Streptococcus sanguis.
Arch Oral Biol. 1969 May;14(5):469-78 PMID: 5255459
Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans.
Odontol Revy. 1970;21(2):143-52 PMID: 4992207
Decreased cariogenicity of a mutant of Streptococcus mutans.
Arch Oral Biol. 1971 Aug;16(8):971-5 PMID: 5284309
Immunization with dextransucrases and glycosidic hydrolases.
J Dent Res. 1972 Mar-Apr;51(2):436-42 PMID: 4501483
Adherence inhibition of Streptococcus mutans: an assay reflecting a possible role of antibody in dental caries prophylaxis.
Infect Immun. 1972 Apr;5(4):419-27 PMID: 4564674

Article Info

Journal

Infection and immunity

Abbr.

Infect Immun

ISSN

0019-9567

DOI

10.1128/iai.7.2.237-241.1973

Published

1973-02-00

Pages

237-41

Language

English

Region

United States

NLM ID

0246127

PMCID

PMC422666

Subset

External Links

PubMed source DOI: 10.1128/iai.7.2.237-241.1973 PMC Full Text View journal details

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Inhibition of glucosyltransferase activity by antisera to known serotypes of Streptococcus mutans.

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