Turbidimetric assay of staphylococcal nuclease.

Applied microbiology ·Vol. 25 ·No. 3 ·1973-03-00 ·Pages 337-41

Erickson A, Deibel RH

Abstract

A simplified turbidimetric procedure was developed to assay staphylococcal nuclease activity. The ease of performance and sensitivity to nanogram quantities enhance the utilization of the method for the quantitative or qualitative estimation of the enzyme. Unlike plating methods, the turbidimetric procedure affords the differentiation between heat-stable and heat-labile nuclease activity.

MeSH Terms

Animals Borates Buffers Cell-Free System Crystallography DNA/metabolism Densitometry Deoxyribonucleases/analysis Evaluation Studies as Topic Hot Temperature Hydrogen-Ion Concentration Hydrolysis Male Methods Micrococcal Nuclease/metabolism Nucleic Acid Denaturation Salmon Spectrophotometry Spermatozoa Staphylococcus/enzymology,growth & development Time Factors

Chemicals

Borates Buffers DNA Deoxyribonucleases Micrococcal Nuclease

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Erickson A

Deibel R H

References (8)

8 references, click to expand

Relationships among coagulase, enterotoxin, and heat-stable deoxyribonuclease production by Staphylococcus aureus.
Appl Microbiol. 1969 Jul;18(1):126-7 PMID: 4896099
Improved medium for detecting deoxyribonuclease-producing bacteria.
Appl Microbiol. 1969 Dec;18(6):991-3 PMID: 4905701
Studies on isomeric dinucleotides derived from deoxyribonucleic acid.
J Biol Chem. 1957 Feb;224(2):751-8 PMID: 13405903
Rapid method for determining the activity of microorganisms on nucleic acids.
J Bacteriol. 1957 Apr;73(4):590-1 PMID: 13428699
Deoxyribonuclease activity of micrococci from clinical sources.
J Bacteriol. 1957 Jun;73(6):747-53 PMID: 13449041
The microdetermination of ribonuclease.
Arch Biochem Biophys. 1958 Feb;73(2):384-90 PMID: 13509723
The turbidimetric determination of deoxyribonuclease activity.
Arch Biochem Biophys. 1959 May;82(1):135-44 PMID: 13650648
Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity.
J Gen Physiol. 1950 Mar;33(4):349-62 PMID: 15406373

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.25.3.337-341.1973

Published

1973-03-00

Pages

337-41

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC380807

Subset

External Links

PubMed source DOI: 10.1128/am.25.3.337-341.1973 PMC Full Text

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