Home LiteratureArticle Details
PMID: 4736109 Published · ppublish English Journal Article

Preparation and properties of an inhibitory extract from frog virus 3 particles.

Journal of virology ·Vol. 11 ·No. 5 ·1973-05-00 ·Pages 694-701

Aubertin AM, Hirth C, Travo C, Nonnenmacher H, Kirn A

Abstract

The structural constituents of the frog virus 3 particle were solubilized by treatment with a nonionic detergent followed by the addition of a high salt concentration. This soluble viral extract (SVE) inhibits host nucleic acid synthesis. Its activity on RNA synthesis was studied in KB cells and found to be dependent on the presence of DEAE dextran. Inactivation of the inhibitory properties of SVE were obtained by trypsin digestion, treatment with urea, or heat denaturation. Neutralization of the activity of SVE was obtained by anti-frog virus 3 serum but not by anti-BHK serum. In vitro a complex may be formed between polynucleotides and the inhibitor indicating a possible mechanism for vivo inhibition.

MeSH Terms
Animals Anura Carcinoma Cell Line Cricetinae DNA Viruses/analysis,enzymology,growth & development Dextrans/pharmacology Hot Temperature Humans Kidney Mouth Neoplasms Neutralization Tests Ornithine/pharmacology Protein Denaturation RNA, Neoplasm/biosynthesis Stereoisomerism Surface-Active Agents Tritium Trypsin/pharmacology Urea/pharmacology Viral Proteins/isolation & purification,pharmacology
Chemicals
Dextrans RNA, Neoplasm Surface-Active Agents Viral Proteins Tritium Urea Ornithine Trypsin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Aubertin A M
Hirth C
Travo C
Nonnenmacher H
Kirn A
References (21)
21 references, click to expand
  1. Viruses and renal carcinoma of Rana pipiens. IV. Nucleic acid synthesis in frog virus 3-infected BHK 21/13 cells.
    Virology. 1967 Nov;33(3):491-502 PMID: 4964866
  2. Chromatographic separation of nucleoside phosphates on diethylaminoethylcellulose paper.
    Anal Biochem. 1968 Jul;24(1):106-11 PMID: 5665189
  3. Effect of poxvirus infection on host cell deoxyribonucleic acid synthesis.
    J Virol. 1968 May;2(5):401-8 PMID: 4301310
  4. Role of adenovirus structural proteins in the cessation of host-cell biosynthetic functions.
    J Virol. 1968 May;2(5):430-9 PMID: 4301313
  5. Deoxyribonucleic acid synthesis in FV-3-infected mammalian cells.
    J Virol. 1968 Oct;2(10):1006-15 PMID: 5749374
  6. Effect of adenovirus on host cell DNA synthesis in synchronized cells.
    Virology. 1969 Apr;37(4):554-64 PMID: 5781860
  7. The step in cellular DNA synthesis blocked by reovirus infection.
    Virology. 1969 Dec;39(4):935-8 PMID: 5358836
  8. The step in cellular DNA synthesis blocked by Newcastle disease or mengovirus infection.
    Virology. 1970 Jan;40(1):152-65 PMID: 4312372
  9. Mechanism by which fiber antigen inhibits multiplication of type 5 adenovirus.
    J Virol. 1967 Aug;1(4):747-57 PMID: 5630225
  10. Selective inhibition of a nuclear RNA polymerase in cells infected with frog virus.
    Nature. 1970 Jul 18;227(5255):294-5 PMID: 5428203
  11. Effect of gamma-rays on infectivity and capacity for nuclear-associated and cytoplasmic DNA replication of FV 3 in chick embryo fibroblasts.
    Radiat Res. 1970 Oct;44(1):178-86 PMID: 5528821
  12. Inhibitory effect of ultraviolet-inactivated vesicular stomatitis virus on initiation o DNA synthesis in cultured chick embryo cells.
    J Gen Virol. 1970 Oct;9(1):69-75 PMID: 4320686
  13. Proteins of polyhedal cytoplasmic deoxyvirus. II. Nucleotide phosphohydrolase activity associated with frog virus 3.
    J Virol. 1971 May;7(5):619-24 PMID: 4327890
  14. Inhibition of host-specific DNA and RNA synthesis in KB cells following infection with frog virus 3.
    J Gen Virol. 1971 Sep;12(3):293-301 PMID: 5165854
  15. Degradation of single- and double-stranded RNA by frog virus 3.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):698-701 PMID: 4335069
  16. Acute hepatitis produced by frog virus 3 in mice. Brief report.
    Arch Gesamte Virusforsch. 1972;36(3):394-7 PMID: 4553583
  17. Viron-associated protein kinase and its involvement in nongenetic reactivation of frog polyhedral cytoplasmic deoxyribovirus.
    Virology. 1972 Jun;48(3):847-51 PMID: 4624201
  18. Activation of the viral genome in Simian Virus 40-transformed nonpermissive cells by permissive cell extracts.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1290-3 PMID: 4338588
  19. Virus-associated DNase: endonuclease in a polyhedral cytoplasmic deoxyvirus.
    Virology. 1972 Jul;49(1):319-21 PMID: 4625022
  20. [Histological and virological study of acute degenerative hepatitis produced by the FV3 (Frog Virus 3) in mice].
    Ann Inst Pasteur (Paris). 1972 Jan;122(1):125-42 PMID: 4625136
  21. [Stimulation of the neutralization ability of FV3 immune serums by fresh guinea pig serum].
    Ann Inst Pasteur (Paris). 1972 Aug;123(2):265-73 PMID: 4120552
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.5.694-701.1973
Published
1973-05-00
Pages
694-701
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355165
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.5.694-701.1973 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2