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PMID: 4758838 Published · ppublish English Journal Article

Regulation of the bacterial cell wall: effect of antibiotics on lipid biosynthesis.

Antimicrobial agents and chemotherapy ·Vol. 4 ·No. 3 ·1973-09-00 ·Pages 346-53

Hebeler BH, Chatterjee AN, Young FE

Abstract

Antibiotics that inhibit the biosynthesis of the cell wall, such as vancomycin, penicillin, d-cycloserine, and bacitracin, stimulate the incorporation of lysine into lipids that are extractable with n-butanol-6 M pyridinium acetate. Approximately 93% of this lysine is in lysylphosphatidylglycerol (LPG). The remaining lysine is incorporated in another as yet uncharacterized lipid. Because the lysine in the latter lipid is released by mild alkaline hydrolysis, it is not the C(55)-isoprenyl-pyrophospho-N-acetyl-muramyl pentapeptide. Vancomycin and penicillin stimulate the incorporation of lysine into both LPG and the minor lipid fractions, whereas treatment with d-cycloserine results in an increase only in LPG. Antibiotics that inhibit protein synthesis do not influence the incorporation of lysine into the lipid fractions. Analysis of the extracted lipids indicate that the incorporation of radioactive lysine into LPG is due to an enhancement in synthesis of LPG from phospholipids in the cytoplasmic membrane.

MeSH Terms
Anti-Bacterial Agents/pharmacology Cell Wall/metabolism Chromatography, Gel Chromatography, Paper Culture Media Glycerophosphates/biosynthesis Hydrolysis Kinetics Lipids/biosynthesis Lysine/metabolism Peptidoglycan/antagonists & inhibitors Phospholipids/biosynthesis Staphylococcus/drug effects,metabolism Tritium
Chemicals
Anti-Bacterial Agents Culture Media Glycerophosphates Lipids Peptidoglycan Phospholipids Tritium Lysine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hebeler B H
Chatterjee A N
Young F E
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29 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.4.3.346
Published
1973-09-00
Pages
346-53
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC444555
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.4.3.346 PMC Full Text View journal details
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