Biosynthesis of serum albumin in rat liver. Evidence for the existence of 'proalbumin'.

The Biochemical journal ·Vol. 134 ·No. 4 ·1973-08-00 ·Pages 1083-91

Judah JD, Gamble M, Steadman JH

Abstract

1. A protein(s) of rat liver (precipitated from soluble extracts of the microsomal fraction by anti-albumin) yields albumin after limited hydrolysis by trypsin. 2. Evidence that the product of limited tryptic hydrolysis is albumin, is based upon ion-exchange chromatography, electrofocusing and peptide ;mapping'. 3. The albumin ;precursor' is recognized by anti-albumin and is apparently not distinguished from albumin by anti-albumin. 4. A small peptide is liberated from the presumptive albumin precursor during limited tryptic hydrolysis. This peptide is labelled by arginine, but not by leucine, lysine or methionine. 5. These results support our previous suggestion based on kinetic evidence that the albumin-like protein(s), in the anti-albumin precipitate from rat liver, is an albumin precursor.

MeSH Terms

Animals Arginine/metabolism Carbon Radioisotopes Chromatography, Ion Exchange Hydrolysis In Vitro Techniques Isoelectric Focusing Liver/metabolism Lysine/metabolism Microsomes, Liver/metabolism Protein Precursors/biosynthesis Rats Serum Albumin/biosynthesis Trypsin/metabolism

Chemicals

Carbon Radioisotopes Protein Precursors Serum Albumin Arginine Trypsin Lysine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Judah J D

Gamble M

Steadman J H

References (15)

15 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1341083

Published

1973-08-00

Pages

1083-91

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1177917

Subset

External Links

PubMed source DOI: 10.1042/bj1341083 PMC Full Text View journal details

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Biosynthesis of serum albumin in rat liver. Evidence for the existence of 'proalbumin'.

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