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PMID: 478564 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The use of agarose in the determination of anchorage-independent growth.

In vitro ·Vol. 15 ·No. 5 ·1979-05-00 ·Pages 351-5

Neugut AI, Weinstein IB

Abstract

At the present time, growth in agar suspension is one of the best in vitro correlates of tumorigenicity. Growth in agarose, however, has not been evaluated extensively as an in vitro criterion for tumorigenicity. In the present study we have tested 19 cell lines, including six mouse-human hybrids, for growth in agarose and agar in the presence and absence of exogenous hypoxanthine. None of the six nontumorigenic cell lines grew in agar or agarose. Ten of the 13 tumorigenic cell lines grew in both agar and agarose with about equal efficiency. The remaining three tumorigenic cell lines grew well in agarose but poorly or not at all in agar. Hypoxanthine did not stimulate the growth in agar or agarose of any of the cell lines except BHK. We conclude that growth in agarose may be a more sensitive marker for tumorigenicity than growth in agar and that BHK is exceptional in its sensitivity to supplemental purines.

MeSH Terms
Agar Animals Cell Division Cell Line Cell Transformation, Neoplastic/pathology Cricetinae HeLa Cells Humans Hybrid Cells Hypoxanthines/metabolism Kidney Mice Neoplasm Transplantation Polysaccharides Sepharose
Chemicals
Hypoxanthines Polysaccharides Agar Sepharose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Neugut A I
Weinstein I B
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Article Info
Journal
In vitro
Abbr.
In Vitro
ISSN
0073-5655
DOI
10.1007/BF02616141
Published
1979-05-00
Pages
351-5
Language
English
Region
United States
NLM ID
0063733
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02616141
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