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PMID: 4790555 Published · ppublish English Journal Article

Correlation between the binding of beta-lactam antibiotics to Staphylococcus aureus and their physical-chemical properties.

Antimicrobial agents and chemotherapy ·Vol. 2 ·No. 3 ·1972-09-00 ·Pages 173-80

Retsema JA, Ray VA

Abstract

The rate of (14)C-benzylpenicillin (penicillin G) binding to Staphylococcus aureus Oxford cells increased with increasing hydrogen ion concentration. The extent of inhibition of (14)C-penicillin G binding caused by a competing (12)C-beta-lactam antibiotic is a function of hydrogen ion concentration and can be correlated both with net charge of a competing (12)C-molecule and net charge of the S. aureus cell at a given pH. The ability of a beta-lactam antibiotic to compete for (14)C-penicillin G-binding sites can generally be correlated with its hydrophobic nature. It is proposed that, although semisynthetic cephalosporins are chemically less reactive than penicillins, they are superior to benzylpenicillin in their ability to permeate the outer surface of the Staphylococcus cell wall and irreversibly bind to reactive sites.

MeSH Terms
Anti-Bacterial Agents/metabolism Binding Sites Carbon Radioisotopes Chemical Phenomena Chemistry Penicillin G/metabolism Staphylococcus/metabolism beta-Lactams/metabolism
Chemicals
Anti-Bacterial Agents Carbon Radioisotopes beta-Lactams Penicillin G
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Retsema J A
Ray V A
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22 references, click to expand
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Article Info
Journal
Antimicrobial agents and chemotherapy
Abbr.
Antimicrob Agents Chemother
ISSN
0066-4804
DOI
10.1128/AAC.2.3.173
Published
1972-09-00
Pages
173-80
Language
English
Region
United States
NLM ID
0315061
PMCID
PMC444286
Subset
IM
External Links
PubMed source DOI: 10.1128/AAC.2.3.173 PMC Full Text View journal details
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