Optimal conditions for transformation of Azotobacter vinelandii.

Journal of bacteriology ·Vol. 139 ·No. 3 ·1979-09-00 ·Pages 1058-61

Page WJ, von Tigerstrom M

Abstract

Optimal transformation of Azotobacter vinelandii OP required a 20-min incubation of the competent cells with deoxyribonucleic acid at 30 degrees C in buffer (pH 6.0 to 8.0) containing 8 mM magnesium sulfate. Nitrogen-fixing transformants of nitrogen fixation-deficient recipients could be plated immediately on selective medium, but transformants acquiring rifampin and streptomycin resistance required preincubation in nonselective medium. The three phenotypes achieved an approximately equal and stable frequency after 17 h (six generations) of growth in nonselective medium.

MeSH Terms

Azotobacter/drug effects,genetics Drug Resistance, Microbial Kinetics Magnesium/pharmacology Nitrogen Fixation Phenotype Rifampin/pharmacology Streptomycin/pharmacology Transformation, Genetic/drug effects

Chemicals

Magnesium Rifampin Streptomycin

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Page W J

von Tigerstrom M

References (15)

15 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.139.3.1058-1061.1979

Published

1979-09-00

Pages

1058-61

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC218056

Subset

External Links

PubMed source DOI: 10.1128/jb.139.3.1058-1061.1979 PMC Full Text View journal details

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Optimal conditions for transformation of Azotobacter vinelandii.

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