Tritium (3H) radiolabeling of protein A and antibody to high specific activity: application to cell surface antigen radioimmunoassays.

Journal of immunological methods ·Vol. 28 ·No. 3-4 ·1979-00-00 ·Pages 255-66

Wilder RL, Yuen CC, Subbarao B, Woods VL, Alexander CB, Mage RG

Abstract

Staphylococcal protein A and several different immunoglobulins have been radiolabeled to high specific activities (greater than 10(6) cpm/microgram) by reductive methylation with tritiated (3H) sodium borohydride. The proteins retain excellent functional and antigenic properties. The utility of these reagents in a variety of assays for cell surface antigens is illustrated. The results indicate that this radiolabeling procedure may become the method of choice for many cell surface and solution immunoassays.

MeSH Terms

Animals Antibodies Antibody Specificity Antigens, Surface Binding Sites, Antibody Borohydrides/pharmacology Goats Immunoglobulin G/metabolism Isotope Labeling Mice Mice, Inbred AKR Mice, Inbred BALB C Mice, Inbred C57BL Mice, Inbred CBA Mice, Inbred DBA Rabbits Radioimmunoassay Staphylococcal Protein A/metabolism Tritium

Chemicals

Antibodies Antigens, Surface Borohydrides Immunoglobulin G Staphylococcal Protein A Tritium

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Wilder R L

Yuen C C

Subbarao B

Woods V L

Alexander C B

Mage R G

Article Info

Journal

Journal of immunological methods

Abbr.

J Immunol Methods

ISSN

0022-1759

DOI

10.1016/0022-1759(79)90192-3

Published

1979-00-00

Pages

255-66

Language

English

Region

Netherlands

NLM ID

1305440

Subset

External Links

PubMed source DOI: 10.1016/0022-1759(79)90192-3 View journal details

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