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PMID: 4796162 Published · ppublish English Journal Article

Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.

Applied microbiology ·Vol. 26 ·No. 3 ·1973-09-00 ·Pages 309-13

Johnson HM, Bukovic JA, Kauffmann PE

Abstract

An immunoassay employing (125)I labeled enterotoxins A and B and polystyrene tubes coated with specific antibodies was used for detection and quantitation of enterotoxin in food. Ham salad, cheddar cheese, custard, condensed milk, and salami were studied. Enterotoxin was successfully determined in all the foods by simple extraction procedures. The assay was sensitive to 1 to 10 ng of toxin per g of food; nonspecific inhibitions were 15% or less.

MeSH Terms
Animals Dairy Products Enterotoxins/analysis Food Analysis Food Contamination Food Microbiology Iodine Radioisotopes Meat Methods Polystyrenes Rabbits/immunology Radioimmunoassay Spectrophotometry Staphylococcus/analysis
Chemicals
Enterotoxins Iodine Radioisotopes Polystyrenes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Johnson H M
Bukovic J A
Kauffmann P E
References (7)
7 references, click to expand
  1. Inhibition of staphylococcal enterotoxin B formation by cell wall blocking agents and other compounds.
    J Bacteriol. 1968 Mar;95(3):1051-5 PMID: 5643046
  2. Standardization of the chloramine-T method of protein iodination.
    Proc Soc Exp Biol Med. 1970 Mar;133(3):989-92 PMID: 4190944
  3. Staphylococcal enterotoxin B: solid-phase radioimmunoassay.
    Appl Microbiol. 1971 Nov;22(5):837-41 PMID: 5002312
  4. Antigenic cross-reactivity of staphylococcal enterotoxins.
    Infect Immun. 1972 May;5(5):645-7 PMID: 4674001
  5. QUANTITATIVE DETECTION OF STAPHYLOCOCCAL ENTEROTOXIN B IN FOOD BY GEL-DIFFUSION METHODS.
    Public Health Rep. 1963 Dec;78:1089-98 PMID: 14090082
  6. THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.
    Biochem J. 1963 Oct;89:114-23 PMID: 14097352
  7. IMMUNOFLUORESCENT DEMONSTRATION OF CELL-ASSOCIATED STAPHYLOCOCCAL ENTEROTOXIN B.
    J Bacteriol. 1965 Apr;89:1155 PMID: 14276112
Article Info
Journal
Applied microbiology
Abbr.
Appl Microbiol
ISSN
0003-6919
Published
1973-09-00
Pages
309-13
Language
English
Region
United States
NLM ID
7605802
PMCID
PMC379780
Subset
IM
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