Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues.

Alanine Amino Acids/analysis Benzoates Carbon Radioisotopes Chemical Phenomena Chemistry Cysteine Cystine Disulfides Electrophoresis, Paper Glutathione Hydrogen-Ion Concentration Indicators and Reagents Kinetics Methods Nitro Compounds Oxidation-Reduction Peptides/analysis Spectrophotometry Sulfhydryl Compounds Thiocyanates

Amino Acids Benzoates Carbon Radioisotopes Disulfides Indicators and Reagents Nitro Compounds Peptides Sulfhydryl Compounds Thiocyanates Cystine Glutathione Cysteine Alanine