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PMID: 482130 Published · ppublish English Journal Article

Modulation of thyroglobulin messenger RNA level by thyrotropin in cultured thyroid cells.

Nucleic acids research ·Vol. 6 ·No. 10 ·1979-07-25 ·Pages 3353-67

Chebath J, Chabaud O, Mauchamp J

Abstract

To examine the influence of thyrotropin (TSH) on the thyroglobulin (Tgb) mRNA content, the latter was evaluated in the cytoplasm of hog thyroid cells cultured in the absence (control cells) or presence of TSH. The Tgb mRNA levels were determined by, (i) kinetics of hybridization to sheep Tgb cDNA, (ii) capacity of coding for peptides immunologically related to Tgb in reticulocyte lysate. In cells cultured for 4 days in the absence of TSH, the content of Tgb mRNA sequences decreased to 30% of its initial value and the messenger activity to 15%. Conversely, TSH maintained the initial Tgb mRNA level in cells cultured in its presence, and TSH concentrations 50 micronU/ml or 5 mU/ml gave identical results. At each period tested poly (A) content was the same in TSH-treated and control cells. When TSH was added to media after 4 or 8 days culture without TSH, the Tgb mRNA level was partially restored. These results suggest that TSH exerts a positive control on Tgb gene expression through modulation of Tgb mRNA content of thyroid cells.

MeSH Terms
Animals Cells, Cultured Kinetics Nucleic Acid Hybridization Polyribosomes/metabolism Protein Biosynthesis RNA, Messenger/metabolism Swine Thyroglobulin/biosynthesis Thyroid Gland/drug effects,metabolism Thyrotropin/pharmacology
Chemicals
RNA, Messenger Thyrotropin Thyroglobulin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chebath J
Chabaud O
Mauchamp J
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43 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.10.3353
Published
1979-07-25
Pages
3353-67
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327938
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.10.3353 PMC Full Text View journal details
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