Human placental cathepsin B1. Isolation and some physical properties.

The Biochemical journal ·Vol. 137 ·No. 2 ·1974-02-00 ·Pages 223-8

Swanson AA, Martin BJ, Spicer SS

Abstract

A reproducible procedure for the isolation, from human placenta, of a cathepsin B1 in a homogeneous state, demonstrated by electrophoretic, ultracentrifugal and enzymic criteria, was carried out. The pH optimum was near pH5.5. The placental enzyme catalysed the release of acid-soluble u.v.-dense products from haemoglobin and myoglobin. It was inhibited by heavy metals and several compounds which react with the thiol groups. The optimum temperature was between 37 degrees and 42 degrees C. The molecular weight of the enzyme was calculated to be 24250.

MeSH Terms

Cathepsins/antagonists & inhibitors,isolation & purification,metabolism Cations, Divalent Chromatography, DEAE-Cellulose Chromatography, Gel Chromatography, Ion Exchange Electrophoresis, Disc Enzyme Activation Female Hemoglobins Humans Hydrogen-Ion Concentration Kinetics Molecular Weight Myoglobin Placenta/enzymology Pregnancy Spectrophotometry, Ultraviolet Sulfhydryl Reagents/pharmacology Temperature Ultracentrifugation Ultrafiltration

Chemicals

Cations, Divalent Hemoglobins Myoglobin Sulfhydryl Reagents Cathepsins

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Swanson A A

Martin B J

Spicer S S

References (14)

14 references, click to expand

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Human cathepsin B1. Purification and some properties of the enzyme.
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Proteinase-catalyzed transamidation and its efficiency.
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J Biol Chem. 1952 Feb;194(2):793-805 PMID: 14927673
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J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
Transamidation reactions catalyzed by cathepsins.
J Biol Chem. 1958 Jan;230(1):1-11 PMID: 13502369
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1370223

Published

1974-02-00

Pages

223-8

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1166108

Subset

External Links

PubMed source DOI: 10.1042/bj1370223 PMC Full Text View journal details

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Human placental cathepsin B1. Isolation and some physical properties.

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