Cytotoxic activity of mouse macrophages studied by various inhibitors.

The Journal of experimental medicine ·Vol. 139 ·No. 5 ·1974-05-01 ·Pages 1049-60

Melsom H

Abstract

The influence of various inhibitors on the cytolytic potential of mouse macrophages against syngeneic erythrocytes has been investigated in vitro by isotope techniques. Intact macrophage membrane and cell metabolism was essential for full cytotoxic activity. The process was completely blocked by anaerobiosis and cold. ATP from both mitochondrial respiration and glycolysis seems to be the high energy intermediate which is utilized during the cytotoxic activity of macrophages leading to target cell lysis. The process did not depend on concomitant DNA transcription, translation, or protein synthesis.

MeSH Terms

Anaerobiosis Animals Antimycin A/pharmacology Chlorpromazine/pharmacology Chromium Radioisotopes Cyanides/pharmacology Cytotoxicity Tests, Immunologic Depression, Chemical Dinitrophenols/pharmacology Erythrocytes/immunology Female Gold/pharmacology Iodoacetates/pharmacology Macrophages/drug effects,immunology Mice Oligomycins/pharmacology Papain/pharmacology Phospholipases/pharmacology Propranolol/pharmacology Surface-Active Agents/pharmacology Tetracaine/pharmacology Thioglycosides/pharmacology Trypsin/pharmacology

Chemicals

Chromium Radioisotopes Cyanides Dinitrophenols Iodoacetates Oligomycins Surface-Active Agents Thioglycosides Tetracaine Antimycin A Gold Propranolol Phospholipases Trypsin Papain Chlorpromazine

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Melsom H

References (13)

13 references, click to expand

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Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.139.5.1049

Published

1974-05-01

Pages

1049-60

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2139660

Subset

External Links

PubMed source DOI: 10.1084/jem.139.5.1049 PMC Full Text View journal details

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Cytotoxic activity of mouse macrophages studied by various inhibitors.

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