Initiation of the replication of single-stranded DNA by concerted action of phage T7 RNA and DNA polymerases.

Adenine Nucleotides/biosynthesis Adenosine Triphosphate/metabolism Bacterial Proteins/pharmacology Centrifugation, Density Gradient Coliphages/enzymology Culture Media DNA Nucleotidyltransferases/metabolism DNA Replication DNA, Circular/biosynthesis DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis DNA-Directed RNA Polymerases/antagonists & inhibitors,metabolism Deoxyribonucleotides/metabolism Guanosine Triphosphate/metabolism Kinetics Polynucleotides/biosynthesis Radioisotopes Ribonucleotides/pharmacology Spermidine/pharmacology Templates, Genetic

Adenine Nucleotides Bacterial Proteins Culture Media DNA, Circular DNA, Single-Stranded DNA, Viral Deoxyribonucleotides Polynucleotides Radioisotopes Ribonucleotides Guanosine Triphosphate Adenosine Triphosphate DNA Nucleotidyltransferases DNA-Directed RNA Polymerases Spermidine