Linkage analysis in Dictyostelium discoideum using temperature-sensitive growth mutants selected with bromodeoxyuridine.

Journal of bacteriology ·Vol. 119 ·No. 3 ·1974-09-00 ·Pages 776-83

Kessin RH, Williams KL, Newell PC

Abstract

Amoebae of the cellular slime mold Dictyostelium discoideum grown in the presence of bromodeoxyuridine are killed on exposure to near-ultraviolet light. By using this phenomenon, a method was devised by which mutants of D. discoideum that are temperature-sensitive for growth can be readily obtained. Three such mutants have been characterized genetically and each was found to be associated with a different linkage group. Two of these linkage groups have not previously been described.

MeSH Terms

Bromodeoxyuridine/pharmacology Cell Survival Chromosome Mapping Diploidy Genetic Linkage Mutagens Mutation Myxomycetes/drug effects,growth & development Nitrosoguanidines Temperature Ultraviolet Rays

Chemicals

Mutagens Nitrosoguanidines Bromodeoxyuridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Kessin R H

Williams K L

Newell P C

References (10)

10 references, click to expand

A proposal for a uniform nomenclature in bacterial genetics.
Genetics. 1966 Jul;54(1):61-76 PMID: 5961488
Developmental regulation of the enzyme UDP-galactose polysaccharide transferase.
Exp Cell Res. 1967 May;46(2):328-34 PMID: 4226096
Genetics of somatic mammalian cells. V. Treatment with 5-bromodeoxyuridine and visible light for isolation of nutritionally deficient mutants.
Proc Natl Acad Sci U S A. 1967 Sep;58(3):1227-34 PMID: 5233843
Temperature-sensitive mutants of Dictyostelium discoideum.
J Bacteriol. 1969 Jul;99(1):65-9 PMID: 5816728
Growth of myxameobae of the cellular slime mould Dictyostelium discoideum in axenic culture.
Biochem J. 1970 Sep;119(2):171-4 PMID: 5530748
Sensitivity of Dictyostelium discoideum to nucleic acid analogues.
Exp Cell Res. 1971 Feb;64(2):484-6 PMID: 5542656
Parasexual recombination in Dictyostelium discoideum: selection of stable diploid heterozygotes and stable haploid segregants (clones-temperature sensitive-ploidy-fruiting bodies-spore-slime mold).
Proc Natl Acad Sci U S A. 1972 Feb;69(2):495-8 PMID: 4501129
Mutagenesis and mutant selection in Physarum polycephalum.
Mol Gen Genet. 1972;118(2):109-24 PMID: 4673170
Genetics of growth in axenic medium of the cellular slime mould Dictyostelium discoideum.
Nature. 1974 Jan 18;247(5437):142-3 PMID: 4855676
An 8-chromosome map of Aspergillus nidulans.
Adv Genet. 1958;9:105-45 PMID: 13520441

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.119.3.776-783.1974

Published

1974-09-00

Pages

776-83

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC245681

Subset

External Links

PubMed source DOI: 10.1128/jb.119.3.776-783.1974 PMC Full Text View journal details

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Linkage analysis in Dictyostelium discoideum using temperature-sensitive growth mutants selected with bromodeoxyuridine.

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