The amino acid sequence of Pseudomonas fluorescens azurin.

The Biochemical journal ·Vol. 104 ·No. 3 ·1967-09-00 ·Pages 784-825

Ambler RP, Brown LH

Abstract

The amino acid sequence of Pseudomonas fluorescens azurin has been determined. The protein consists of a single peptide chain of 128 residues. There is one intra-chain disulphide bridge. The sequence was determined by isolation of the soluble tryptic peptides, and by exhaustive examination of the products of chymotryptic and peptic digestion. The sequence has been confirmed by the purification and analysis of the seven fragments obtained by cyanogen bromide treatment of the protein.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/analysis Binding Sites Carboxypeptidases/metabolism Chromatography, Gel Chromatography, Paper Chymotrypsin/metabolism Copper Electrophoresis Pepsin A/metabolism Peptides/analysis Pseudomonas/analysis Spectrum Analysis

Chemicals

Bacterial Proteins Peptides Copper Carboxypeptidases Chymotrypsin Pepsin A

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Ambler R P

Brown L H

References (18)

18 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1040784

Published

1967-09-00

Pages

784-825

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1271220

Subset

External Links

PubMed source DOI: 10.1042/bj1040784 PMC Full Text View journal details

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The amino acid sequence of Pseudomonas fluorescens azurin.

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