Microplate enzyme-linked immunosorbent assay for chagas' disease.

Lancet (London, England) ·Vol. 1 ·No. 7904 ·1975-02-22 ·Pages 426-8

Voller A, Draper C, Bidwell DE, Bartlett A

Abstract

A microscale immunosorbent assay (E.L.I.S.A.) for the measurement of antibody in Chagas' disease (American trypanosomiasis) is described. The test was assessed on plasma samples from people living in Bahia, Brazil. 98% of the individuals with positive Trypanosoma cruzi immunofluorescent-antibody (I.F.A.) tests gave E.L.I.S.A. reading considered as positive. Less than 2% of those with negative I.F.A. tests had positive E.L.I.S.A. reactions. This simple test may be suitable for use in large seroepidemiological surveys.

MeSH Terms

Adsorption Alkaline Phosphatase Animals Antibodies/isolation & purification Blood Specimen Collection Brazil Chagas Disease/diagnosis,immunology,microbiology False Negative Reactions False Positive Reactions Fluorescent Antibody Technique Humans Immunoassay Immunoglobulin G Immunoglobulins Rabbits/immunology Serologic Tests Spectrophotometry Trypanosoma cruzi/immunology

Chemicals

Antibodies Immunoglobulin G Immunoglobulins Alkaline Phosphatase

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Voller A

Draper C

Bidwell D E

Bartlett A

Article Info

Journal

Lancet (London, England)

Abbr.

Lancet

ISSN

0140-6736

DOI

10.1016/s0140-6736(75)91492-0

Published

1975-02-22

Pages

426-8

Language

English

Region

England

NLM ID

2985213R

Subset

External Links

PubMed source DOI: 10.1016/s0140-6736(75)91492-0 View journal details

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