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PMID: 4862195 Published · ppublish English Journal Article

Urea production and putrescine biosynthesis by Escherichia coli.

Journal of bacteriology ·Vol. 94 ·No. 5 ·1967-11-00 ·Pages 1516-9

Morris DR, Koffron KL

Abstract

Cultures of Escherichia coli B and K-12 produce urea during growth in minimal medium. Results of isotopic labeling experiments were consistent with the sole source of urea being from the conversion of arginine to putrescine. Since E. coli itself has no demonstrable urease activity, the rate of urea production is a measure of the flux through the arginine to putrescine pathway.

MeSH Terms
Amines/biosynthesis Arginine/metabolism Carbon Isotopes Citrulline/metabolism Escherichia coli/enzymology,metabolism Glucose/metabolism Ornithine/metabolism Urea/biosynthesis Urease/metabolism
Chemicals
Amines Carbon Isotopes Citrulline Urea Arginine Ornithine Urease Glucose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Morris D R
Koffron K L
References (7)
7 references, click to expand
  1. A biosynthetic ornithine decarboxylase in Escherichia coli.
    Biochem Biophys Res Commun. 1965 Sep 22;20(6):697-702 PMID: 5324102
  2. Multiple pathways of putrescine biosynthesis in Escherichia coli.
    J Biol Chem. 1966 Jul 10;241(13):3129-35 PMID: 5330264
  3. Simplified tests for some amino acid decarboxylases and for the arginine dihydrolase system.
    Acta Pathol Microbiol Scand. 1955;36(2):158-72 PMID: 14375937
  4. Reversible specific concentration of amino acids in Escherichia coli.
    Ann Inst Pasteur (Paris). 1956 Nov;91(5):693-720 PMID: 13395009
  5. The enzymology of control by feedback inhibition.
    J Biol Chem. 1962 Mar;237:891-6 PMID: 13897943
  6. The genetic control of the enzymes of histidine biosynthesis in Salmonella typhimurium.
    J Gen Microbiol. 1960 Apr;22:369-78 PMID: 13793162
  7. The acetylation of polyamines in Escherichia coli.
    J Biol Chem. 1960 Mar;235:776-82 PMID: 13818299
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
Published
1967-11-00
Pages
1516-9
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC276857
Subset
IM
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