An assay method for leukocyte pyrogen.

The Journal of experimental medicine ·Vol. 126 ·No. 5 ·1967-11-01 ·Pages 745-61

Murphy PA

Abstract

A method for assaying leukocyte pyrogen is described which is shown to remain valid despite the presence of very large amounts of bacterial endotoxin. It uses a combination of two procedures to inactivate endotoxin: incubation in normal rabbit plasma for 24 hr at 37 degrees C, and injection into rabbits rendered refractory to endotoxin. The validity of the assay was confirmed by formal statistical methods. A simplified assay method is also described which lacks full statistical validity, but is more suitable for routine use.

MeSH Terms

Animals Biological Assay Endotoxins/pharmacology Escherichia coli In Vitro Techniques Leukocytes/analysis Proteus Pyrogens/administration & dosage,blood Rabbits

Chemicals

Endotoxins Pyrogens

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Murphy P A

References (8)

8 references, click to expand

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J Exp Med. 1963 Mar 1;117:349-64 PMID: 14014022
Inactivation of endotoxin by a humoral component. II. Interaction of endotoxin with serum and plasma.
J Exp Med. 1958 Nov 1;108(5):685-99 PMID: 13587851
Studies on the pathogenesis of fever. I. The presence of transferable pyrogen in the blood stream following the injection of typhoid vaccine.
J Exp Med. 1955 May 1;101(5):519-28 PMID: 14367676
Observations on the production of pyrogenic substances by rabbit and human leucocytes.
J Exp Med. 1961 Jun 1;113:1127-40 PMID: 13699218
The initial process in the action of bacterial pyrogens in man.
Clin Sci (Lond). 1954 Nov;13(4):453-9 PMID: 13209831
Studies on the pathogenesis of fever. II. Identification of an endogenous pyrogen in the blood stream following the injection of typhoid vaccine.
J Exp Med. 1955 Nov 1;102(5):499-516 PMID: 13271667
Studies on the pathogenesis of fever. VI. The interaction of leucocytes and endotoxin in vitro.
J Exp Med. 1959 Dec 1;110:1005-16 PMID: 13811296
Inactivation of endotoxin by a humoral component. III. Role of divalent cation and a dialyzable component.
J Exp Med. 1958 Nov 1;108(5):701-11 PMID: 13587852

Article Info

Journal

The Journal of experimental medicine

Abbr.

J Exp Med

ISSN

0022-1007

DOI

10.1084/jem.126.5.745

Published

1967-11-01

Pages

745-61

Language

English

Region

United States

NLM ID

2985109R

PMCID

PMC2138405

Subset

External Links

PubMed source DOI: 10.1084/jem.126.5.745 PMC Full Text View journal details

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An assay method for leukocyte pyrogen.

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