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PMID: 4867214 Published · ppublish English Journal Article

Evidence for a relationship between deoxyribonucleic acid metabolism and septum formation in Escherichia coli.

Journal of bacteriology ·Vol. 95 ·No. 1 ·1968-01-00 ·Pages 123-31

Walker JR, Pardee AB

Abstract

Septum formation is a key step in bacterial division, but the mechanism which controls periodic septum formation is unknown. In an attempt to understand this mechanism, lon(-) mutants, in which septum formation is blocked by very doses of ultraviolet light (UV), were investigated. UV must act on some part of the apparatus of cytokinesis; thus, identification of the UV target would identify part of this apparatus. As likely possibilities, UV might damage the septum-forming site or it might damage deoxyribonucleic acid (DNA), since DNA replication is normally coordinated with septum formation. To distinguish between these possibilities, DNA was specifically sensitized by incorporating bromodeoxyuridine into lon(-) bacteria. These bacteria were strongly sensitized to longer wavelength UV (2,900 to 3,100 A) so that they failed to form septa, grew into filaments which lysed, and did not form colonies. Various control experiments supported the conclusion that UV inhibits septum formation as a result of alterations in DNA metabolism. A relationship thus exists between DNA metabolism and septum formation.

MeSH Terms
Bromodeoxyuridine/pharmacology Carbon Isotopes Cell Division DNA, Bacterial/metabolism,radiation effects Escherichia coli/metabolism,radiation effects Models, Theoretical Mutation Thymidine/metabolism Thymine/metabolism Ultracentrifugation Ultraviolet Rays
Chemicals
Carbon Isotopes DNA, Bacterial Bromodeoxyuridine Thymine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Walker J R
Pardee A B
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32 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.95.1.123-131.1968
Published
1968-01-00
Pages
123-31
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC251980
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.95.1.123-131.1968 PMC Full Text View journal details
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