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PMID: 4868351 Published · ppublish English Journal Article

Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation.

Journal of bacteriology ·Vol. 95 ·No. 3 ·1968-03-00 ·Pages 1040-50

Boyd D, Nixon R, Gillespie S, Gillespie D

Abstract

A system for screening Escherichia coli temperature-sensitive mutants is described. The system involves glucose starvation and minimizes ambiguities introduced by the interdependencies of macromolecular synthesis during balanced growth. The system permits the quick recognition of protein synthesis mutants and their classification into two general catagories. Complete protein synthesis mutants are unable to make any polypeptide material, whereas partial protein synthesis mutants are able to produce inactive proteins. The phenotypes of several mutants are described.

MeSH Terms
Bacterial Proteins/biosynthesis Carbon Isotopes Coliphages/growth & development Escherichia coli/metabolism Galactosidases/biosynthesis Genetics, Microbial Glucose/metabolism Leucine/metabolism Lysogeny Mutation Temperature Thymidine/metabolism Tritium Uridine/metabolism
Chemicals
Bacterial Proteins Carbon Isotopes Tritium Galactosidases Leucine Glucose Thymidine Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boyd D
Nixon R
Gillespie S
Gillespie D
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25 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.95.3.1040-1050.1968
Published
1968-03-00
Pages
1040-50
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC252129
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.95.3.1040-1050.1968 PMC Full Text View journal details
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