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PMID: 4874495 Published · ppublish English Journal Article

Ultrastructure of human leukocytes after simultaneous fixation with glutaraldehyde and osmium tetroxide and "postfixation" in uranyl acetate.

The Journal of cell biology ·Vol. 38 ·No. 3 ·1968-09-00 ·Pages 615-27

Hirsch JG, Fedorko ME

Abstract

Human leukocytes in suspension or in monolayer cultures have been processed for electron microscopy by fixation in a freshly made cold mixture of glutaraldehyde and osmium tetroxide and by "postfixation" in uranyl acetate. Simultaneous exposure to glutaraldehyde and osmium tetroxide eliminates many of the shortcomings seen when either of these agents is used alone as the initial fixative. Specimens are processed to the stage of dehydration as single cell suspensions or as very small clumps to assure rapid penetration of fixatives and efficient washing. The technique is rapid and reproducible. Electron micrographs presented in this report illustrate the ultrastructural features of human white cells prepared by this method.

MeSH Terms
Acetates Aldehydes Culture Techniques Histological Techniques Humans Leukocytes/cytology Methods Microscopy, Electron Osmium Uranium
Chemicals
Acetates Aldehydes Osmium Uranium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hirsch J G
Fedorko M E
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.38.3.615
Published
1968-09-00
Pages
615-27
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2108377
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.38.3.615 PMC Full Text View journal details
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