Extraction and isolation of individual ribosomal proteins from Escherichia coli.

Journal of bacteriology ·Vol. 96 ·No. 2 ·1968-08-00 ·Pages 358-64

Fogel S, Sypherd PS

Abstract

We have described a new method for the quantitative separation of ribosomal proteins and ribosomal ribonucleic acid. A procedure for the preparation of individual ribosomal proteins by polyacrylamide gel electrophoresis is also described. By the use of gels with smaller pores, at least four of the electrophoretic components from the 30S ribosome can be split into additional protein fractions. By the methods described here, it is possible to isolate in high purity at least 15 different proteins from the 30S ribosome of Escherichia coli.

MeSH Terms

Bacterial Proteins/analysis Centrifugation, Density Gradient Chemical Precipitation Densitometry Electrophoresis, Disc/instrumentation Escherichia coli/analysis Methods RNA, Bacterial/analysis Ribosomes/analysis

Chemicals

Bacterial Proteins RNA, Bacterial

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Fogel S

Sypherd P S

References (15)

15 references, click to expand

FRACTIONATION OF THE RIBOSOMAL PROTEIN FROM ESCHERICHIA COLI.
J Mol Biol. 1964 Nov;10:319-36 PMID: 14235590
The requirements for specific sRNA binding by ribosomes.
J Mol Biol. 1966 Jun;18(1):90-108 PMID: 5337561
Isolation and characterization of ribonuclease I mutants of Escherichia coli.
J Mol Biol. 1966 Mar;16(1):67-84 PMID: 5331244
Ribosomal proteins of Escherichia coli. I. Demonstration of different primary structures.
Proc Natl Acad Sci U S A. 1967 May;57(5):1294-301 PMID: 5341237
Studies on the composition of the protein from Escherichia coli ribosomes.
Proc Natl Acad Sci U S A. 1961 Jan 15;47:18-23 PMID: 13782837
CHLORAMPHENICOL-PROMOTED REPRESSION OF beta-GALACTOSIDASE SYNTHESIS IN ESCHERICHIA COLI.
Proc Natl Acad Sci U S A. 1963 Mar;49(3):400-7 PMID: 16591059
Chemical basis for heterogeneity of ribosomal proteins.
Proc Natl Acad Sci U S A. 1968 Apr;59(4):1329-36 PMID: 4870863
Disk electrophoresis of basic proteins and peptides on polyacrylamide gels.
Nature. 1962 Jul 21;195:281-3 PMID: 14491328
Fractionation and some properties of the ribosomal proteins in Escherichia coli ribosomes.
Biochim Biophys Acta. 1967 Dec 12;147(3):427-40 PMID: 4866585
Amino-acid sequence of horse heart cytochrome c.
Nature. 1961 Dec 23;192:1125-7 PMID: 14469771
Ribosomal proteins of Escherichia coli. II. Proteins from the 30 s subunit.
J Mol Biol. 1968 Feb 14;31(3):441-61 PMID: 4866333
THE PREPARATION OF RIBOSOMAL PROTEIN FROM ESCHERICHIA COLI WITH LITHIUM CHLORIDE AND UREA.
Biochem Biophys Res Commun. 1965 Feb 17;18:557-62 PMID: 14301460
Ribosomal proteins. I. Isolation, amino acid composition, molecular weights and peptide mapping of proteins from E. coli ribosomes.
Mol Gen Genet. 1967;100(4):364-73 PMID: 4869077
Primary structure heterogeneity in ribosomal proteins from Escherichia coli.
Nature. 1967 Sep 16;215(5107):1293-5 PMID: 4861196
Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.96.2.358-364.1968

Published

1968-08-00

Pages

358-64

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC252306

Subset

External Links

PubMed source DOI: 10.1128/jb.96.2.358-364.1968 PMC Full Text View journal details

Analysis Services

Extraction and isolation of individual ribosomal proteins from Escherichia coli.

Contact

WeChat Official Account