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PMID: 4880718 Published · ppublish English Journal Article

Nonspecific staining: its control in immunofluorescence examination of soil.

Science (New York, N.Y.) ·Vol. 162 ·No. 3857 ·1968-11-29 ·Pages 1012-4

Bohlool BB, Schmidt EL

Abstract

Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.

MeSH Terms
Animals Fluorescent Antibody Technique Fluorescent Dyes Francisella tularensis/immunology Gelatin Liver/pathology Mice Pasteurella Infections/pathology Rhizobium/immunology Soil Microbiology Spleen/pathology Thiocyanates
Chemicals
Fluorescent Dyes Thiocyanates Gelatin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bohlool B B
Schmidt E L
Article Info
Journal
Science (New York, N.Y.)
Abbr.
Science
ISSN
0036-8075
DOI
10.1126/science.162.3857.1012
Published
1968-11-29
Pages
1012-4
Language
English
Region
United States
NLM ID
0404511
Subset
IM
External Links
PubMed source DOI: 10.1126/science.162.3857.1012 View journal details
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