Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies.

Applied microbiology ·Vol. 16 ·No. 11 ·1968-11-00 ·Pages 1640-5

Zimmerman RA, Mathews J, Wilson E

Abstract

A number of investigators have attempted to utilize the hemagglutination system for detection of streptococcal type-specific antibody in human sera. Cross-reactions have made the procedure unreliable without cumbersome and time-consuming manipulation of the test sera. A method is described in which a microtiter indirect hemagglutination technique, using sensitized sheep erythrocytes, is sensitive, specific, and reliable for titration of type-specific antibody after naturally acquired or induced streptococcal infection.

MeSH Terms

Animals Antibodies/analysis Antigen-Antibody Reactions Bacterial Proteins Blood Bactericidal Activity Erythrocytes Hemagglutination Inhibition Tests Hemagglutination Tests Hominidae Humans Immune Sera Methods Rabbits Sheep Streptococcal Infections/immunology Streptococcus pyogenes/immunology

Chemicals

Antibodies Bacterial Proteins Immune Sera

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Zimmerman R A

Mathews J

Wilson E

References (10)

10 references, click to expand

The demonstration of type specific streptococcal antibody by a hemagglutination technique employing tannic acid.
J Clin Invest. 1953 Nov;32(11):1085-93 PMID: 13108970
STREPTOCOCCAL M PROTEIN ANTIBODIES.
J Infect Dis. 1963 Jul-Aug;113:33-8 PMID: 14044090
THE MEASUREMENT OF TYPE- AND NONTYPE-SPECIFIC GROUP A HEMOLYTIC STREPTOCOCCAL ANTIBODY WITH AN HEMAGGLUTINATION TECHNIQUE.
J Immunol. 1964 Feb;92:185-91 PMID: 14160438
Long chain formation by strains of group A streptococci in the presence of homologous antiserum: a type-specific reaction.
J Exp Med. 1957 Sep 1;106(3):345-56 PMID: 13463246
Mouse protection and enhancement of phagocytosis by antisera to Streptococcus pyogenes.
Br J Exp Pathol. 1956 Aug;37(4):406-14 PMID: 13364151
Differentiation of group A streptococci with a common R antigen into three serological types, with special reference to the bactericidal test.
J Exp Med. 1957 Oct 1;106(4):525-44 PMID: 13475611
The indirect bactericidal test as a means of identifying antibody to the M antigen of Streptococcus pyogenes.
Br J Exp Pathol. 1956 Aug;37(4):415-22 PMID: 13364152
Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus.
J Exp Med. 1952 Jul;96(1):71-82 PMID: 14946330
Current knowledge of type-specific M antigens of group A streptococci.
J Immunol. 1962 Sep;89:307-13 PMID: 14461914
Type specific antibodies to Streptococcus pyogenes.
Aust J Exp Biol Med Sci. 1958 Aug;36(4):395-403 PMID: 13607347

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.16.11.1640-1645.1968

Published

1968-11-00

Pages

1640-5

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC547732

Subset

External Links

PubMed source DOI: 10.1128/am.16.11.1640-1645.1968 PMC Full Text

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Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies.

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