The sequence of amino acids in insulin isolated from islet tissue of the cod (Gadus callarias).

The Biochemical journal ·Vol. 110 ·No. 2 ·1968-11-00 ·Pages 289-96

Reid KB, Grant PT, Youngson A

Abstract

1. S-Aminoethylcysteinyl derivatives of the A and B chains of cod insulin were prepared from the individual S-sulpho chains. 2. Studies on small peptides derived from the S-aminoethylated peptide chains by treatment with trypsin allowed the amino acid sequences in the region of the cysteinyl residues of the A and B peptide chains to be defined. 3. The six amide groups in cod insulin were located by complete digestion of small peptides from the A and B chains with aminopeptidase followed by amino acid analyses. 4. The results, together with previous studies on the oxidized A and B chains, define the sequences of the 51 amino acids that constitute cod insulin.

MeSH Terms

Amino Acid Sequence Amino Acids/analysis Aminopeptidases Animals Fishes Insulin/analysis Islets of Langerhans/analysis Peptides/metabolism

Chemicals

Amino Acids Insulin Peptides Aminopeptidases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Reid K B

Grant P T

Youngson A

References (32)

32 references, click to expand

The structure of pig and sheep insulins.
Biochem J. 1955 Aug;60(4):556-65 PMID: 13249948
THE NH2-TERMINAL RESIDUES OF THE PROTEINS FROM CELL-FREE EXTRACTS OF E. COLI.
J Mol Biol. 1963 Nov;7:483-96 PMID: 14079588
THE COMPLETE AMINO ACID SEQUENCE OF THE TRYPTOPHAN SYNTHETASE A PROTEIN (alpha SUBUNIT) AND ITS COLINEAR RELATIONSHIP WITH THE GENETIC MAP OF THE A GENE.
Proc Natl Acad Sci U S A. 1967 Feb;57(2):296-8 PMID: 16591468
Coding response of N-fromyl-methionyl-sRNA to UUG.
Nature. 1965 Sep 4;207(5001):1038-9 PMID: 5325492
A convenient preparation of reduced and S-sulfonated A and B chains of insulin.
Biochim Biophys Acta. 1966 Sep 26;127(1):246-9 PMID: 5970879
The sequence of the amino acid residues in performic acid-oxidized ribonuclease.
J Biol Chem. 1960 Mar;235:633-47 PMID: 14401957
Species-specificity of human antibeef, pork insulin serum.
J Clin Invest. 1959 Nov;38:2017-25 PMID: 13799924
Modification of methionyl residues during aminoethylation.
Biochim Biophys Acta. 1967 Dec 12;147(3):590-2 PMID: 6081858
THE AMINO ACID SEQUENCE OF THE GAMMA CHAIN OF HUMAN FETAL HEMOGLOBIN.
Biochemistry. 1963 Sep-Oct;2:992-1008 PMID: 14087393
Isolation and a partial amino acid sequence of insulin from the islet tissue of cod (Gadus callarias).
Biochem J. 1968 Jan;106(2):531-41 PMID: 4866431
Species differences in insulin.
Arch Biochem Biophys. 1956 Nov;65(1):427-38 PMID: 13373434
The synthesis of arginyl dipeptides; the action of papain and trypsin.
J Biol Chem. 1954 Jun;208(2):751-64 PMID: 13174584
Isolation and partial structural analysis of insulin from the separate islet tissue of Lophius piscatorius.
Biochemistry. 1965 Jun;4(6):1044-8 PMID: 5320386
Species variation in the amino acid sequence of insulin.
Am J Med. 1966 May;40(5):662-6 PMID: 5949593
[Insulin from the hagfish (Myxine glutinosa: Cyclostomata)].
Hoppe Seylers Z Physiol Chem. 1967 May;348(5):525-32 PMID: 5586898
THE AMINO ACID SEQUENCE OF EGG WHITE LYSOZYME.
J Biol Chem. 1963 Aug;238:2698-707 PMID: 14063294
The antigenic loci of insulin.
Can J Biochem. 1967 Jul;45(7):1135-44 PMID: 6035974
Regeneration of insulin activity from the separated and inactive A and B chains.
Nature. 1960 Nov 26;188:721-4 PMID: 13723142
A comparison of amino acid sequences in the beta-chains of adult bovine hemoglobins A and B.
Arch Biochem Biophys. 1967 Apr;120(1):124-35 PMID: 6048711
Studies on insulin. III. On the structure of the alanyl chain of bonito insulin.
J Biochem. 1962 Apr;51:301-9 PMID: 14036898
The tryptic digestion of S-aminoethylated ribonuclease.
J Biol Chem. 1967 Jan 25;242(2):265-70 PMID: 6016611
Relationship of structure to biological activity of insulin as revealed by degradative studies.
Am J Med. 1966 May;40(5):750-8 PMID: 5328875
INHIBITION OF CHYMOTRYPSIN ACTIVITY IN CRYSTALLINE TRYPSIN PREPARATIONS.
J Biol Chem. 1964 Jun;239:1799-803 PMID: 14213354
A comparison of cod and bovine insulins.
Nature. 1961 Aug 26;191:876-9 PMID: 13785708
The complete enzymic hydrolysis of proteins.
J Biol Chem. 1962 Feb;237:389-96 PMID: 13907377
Comparative aspects of the immunology and biology of insulin.
Diabetologia. 1967 Dec;3(6):519-28 PMID: 5620812
Studies on pituitary polypeptide hormones. II. The action of pepsin and subtilisin on beta-melanocyte-stimulating hormone.
Biochem J. 1959 Mar;71(3):445-51 PMID: 13638249
The catalytic nature of insulin.
Am J Med. 1966 May;40(5):759-64 PMID: 5941969
Cyanogen bromide cleavage and partial sequence of the heavy chain of a pathological immunoglobulin G.
Biochem J. 1967 Aug;104(2):616-26 PMID: 4167412
Differences in the amino acid sequences of tryptic peptides from three sheep hemoglobin beta chains.
J Biol Chem. 1967 May 10;242(9):2211-32 PMID: 6022868
Sub-unit structure and specificity of methionyl-transfer-ribonucleic acid synthetase from Escherichia coli.
Biochem J. 1968 Jun;108(2):281-8 PMID: 4874971
On the aminoethylation of proteins.
J Biol Chem. 1966 Aug 10;241(15):3457-61 PMID: 5919679

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1100289

Published

1968-11-00

Pages

289-96

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1187208

Subset

External Links

PubMed source DOI: 10.1042/bj1100289 PMC Full Text View journal details

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The sequence of amino acids in insulin isolated from islet tissue of the cod (Gadus callarias).

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