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PMID: 4884816 Published · ppublish English Journal Article

Genetic analysis of carbohydrate transport-deficient mutants of Salmonella typhimurium.

Journal of bacteriology ·Vol. 97 ·No. 1 ·1969-01-00 ·Pages 250-5

Levinthal M, Simoni RD

Abstract

Mutants (car) isolated from Salmonella typhimurium were unable to utilize or ferment the following carbohydrates (all d-configuration): glucose, fructose, mannose, N-acetylglucosamine, sorbitol, mannitol, maltose, melibiose, and glycerol. The mutants did utilize galactose, glucose 6-phosphate, gluconic acid, glucuronic acid, pyruvate, and l-lactate. Biochemical analysis showed that there were two classes of mutants, each lacking one component of a phosphotransferase system. CarA mutants were deficient in enzyme I; carB lacked the phosphate carrier protein, HPr. Mapping experiments showed that the carA gene was located near pro; the carB gene mapped near purC.

MeSH Terms
Biological Transport Carbohydrate Metabolism Chromosome Mapping Disaccharides/metabolism Fermentation Fructose/metabolism Galactose/metabolism Genetics, Microbial Gluconates/metabolism Glucosamine/metabolism Glucose/metabolism Glucuronates/metabolism Glycerol/metabolism Hexosephosphates/metabolism Lactates/metabolism Maltose/metabolism Mannitol/metabolism Mannose/metabolism Mutation Phosphotransferases/biosynthesis Pyruvates/metabolism Salmonella typhimurium/metabolism Sorbitol/metabolism
Chemicals
Disaccharides Gluconates Glucuronates Hexosephosphates Lactates Pyruvates Fructose Mannitol Sorbitol Maltose Phosphotransferases Glucose Glucosamine Glycerol Mannose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Levinthal M
Simoni R D
References (14)
14 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.97.1.250-255.1969
Published
1969-01-00
Pages
250-5
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC249589
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.97.1.250-255.1969 PMC Full Text View journal details
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