Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.
Three strains of Escherichia coli with altered polynucleotide phosphorylase, Q7, Q13, and Q27, were isolated by screening clones from heavily mutagenized cultures for low levels of the enzyme. The three mutations were found to cotransduce with argG and asp, and the pnp locus which they define was mapped with respect to these loci. An explanation for the nonreciprocal cotransduction frequencies observed with asp is provided by the demonstration of an unlinked asp-suppressing locus.
17 references, click to expand
-
Specific template requirments of RNA replicases.Proc Natl Acad Sci U S A. 1965 Aug;54(2):579-87 PMID: 5217445
-
Isolation and characterization of ribonuclease I mutants of Escherichia coli.J Mol Biol. 1966 Mar;16(1):67-84 PMID: 5331244
-
Synthesis and breakdown of ribonucleic acid in Escherichia coli starving for nitrogen.Biochim Biophys Acta. 1966 Apr 18;119(1):183-91 PMID: 5335523
-
Translation of the genetic message: factors involved in the initiation of protein synthesis.Proc Natl Acad Sci U S A. 1966 Jul;56(1):290-5 PMID: 5338590
-
On the metabolic inactivation of messenger RNA in Escherichia coli: ribonuclease I and polynucleotide phosphorylase.Biochim Biophys Acta. 1967 Mar 29;138(1):66-75 PMID: 4860430
-
An altered polynucleotide phosphorylase in E. coli mutant Q13.Biochem Biophys Res Commun. 1967 Aug 7;28(3):374-9 PMID: 4861585
-
Turnover of ribosomal RNA in a ribonuclease I-less mutant of Escherichia coli, Q-13, which was found to possess polynucleotide phosphorylase.Biochim Biophys Acta. 1967 Sep 26;145(2):328-36 PMID: 4294276
-
The preparation, properties, and reactions of succinyl coenzyme A synthetase and its phosphorylated form.J Biol Chem. 1967 Oct 10;242(19):4287-98 PMID: 4863737
-
Inhibition by oligonucleotides of the autodegradation of ribosomal and messenger RNA in cell-free preparations from ribonuclease I-less bacteria.Biochim Biophys Acta. 1967;145(3):621-8 PMID: 4863904
-
Measurement of the unstable RNA in exponentially growing cultures of Bacillus subtilis and Escherichia coli.J Mol Biol. 1968 Jan 28;31(2):237-66 PMID: 4965769
-
Revised linkage map of Escherichia coli.Bacteriol Rev. 1967 Dec;31(4):332-53 PMID: 4865540
-
Purification and properties of polynucleotide phosphorylase from Escherichia coli.J Biol Chem. 1968 Jan 25;243(2):231-40 PMID: 4866865
-
Polymerization of nucleoside diphosphate with a manganese-dependent enzyme from Escherichia coli Q13.Proc Natl Acad Sci U S A. 1967 Dec;58(6):2468-75 PMID: 4873592
-
Mutagenesis of the replication point by nitrosoguanidine: map and pattern of replication of the Escherichia coli chromosome.J Mol Biol. 1968 May 14;33(3):705-19 PMID: 4882616
-
Characterization of polynucleotide phosphorylase mutants of Escherichia coli.J Bacteriol. 1969 Mar;97(3):1437-43 PMID: 4887520
-
Genetic locus for ribonuclease I in Escherichia coli.J Bacteriol. 1969 Mar;97(3):1522-3 PMID: 4887525
-
Hydrolysis of oligoribonucleotides by an enzyme fraction from Escherichia coli.Biochem Biophys Res Commun. 1967 Nov 30;29(4):550-5 PMID: 16496534
Contact
No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong
Qilu Normal University · Genelibs Bioinformatics Lab
750 Shunhua Rd, Jinan
2F, Bldg F, University Science Park
Tel: 0531-88819269
WeChat Official Account
Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.
Business Email
E-mail: [email protected]
Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2
