The influence of culture medium composition on the incidence of chromosomal breakage.

Human genetics ·Vol. 50 ·No. 3 ·1979-09-00 ·Pages 277-83

Keck M, Emerit I

Abstract

In patients with chromosomal instability and healthy subjects, significant differences were observed in the chromosomal breakage incidence in simultaneous lymphocyte cultures set up with TC medium 199, Eagle's Minimum Essential Medium, or RPMI, or RPMI 1629. The importance of the choice of culture medium for mutagenicity testing and studies of so-called spontaneous breakage is shown. Cultures incubated with TC Medium 199 showed the highest chromosomal breakage incidence.

MeSH Terms

Ascorbic Acid Cells, Cultured Chromosome Aberrations/pathology Chromosome Disorders Chromosomes, Human/ultrastructure Culture Media Cysteine Humans Lymphocytes/physiology Polysorbates

Chemicals

Culture Media Polysorbates Cysteine Ascorbic Acid

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Keck M

Emerit I

References (10)

10 references, click to expand

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Article Info

Journal

Human genetics

Abbr.

Hum Genet

ISSN

0340-6717

DOI

10.1007/BF00399393

Published

1979-09-00

Pages

277-83

Language

English

Region

Germany

NLM ID

7613873

Subset

External Links

PubMed source DOI: 10.1007/BF00399393 View journal details

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The influence of culture medium composition on the incidence of chromosomal breakage.

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