Tube dilution antimicrobial susceptibility testing: efficacy of a microtechnique applicable to diagnostic laboratories.

Applied microbiology ·Vol. 17 ·No. 5 ·1969-05-00 ·Pages 707-9

Chitwood LA

Abstract

A microtechnique for determining antibiotic susceptibilities by the serial dilution method was evaluated in a clinical diagnostic microbiology laboratory. As compared with the standard tube method, an agreement of 94% was achieved for determining minimal inhibitory concentration with +/- one tube dilution as the criterion of comparison. The experience with this system suggests that it could easily be incorporated into diagnostic laboratories as a routine procedure.

MeSH Terms

Anti-Bacterial Agents Bacteria/drug effects Bacteriological Techniques Drug Resistance, Microbial Methods

Chemicals

Anti-Bacterial Agents

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Chitwood L A

References (9)

9 references, click to expand

Methods and significance of in vitro testing of bacterial sensitivity to drugs.
Am J Med. 1965 Nov;39(5):766-79 PMID: 5319390
Application of microtitration techniques to bacteriostatic and bactericidal antibiotic susceptibility testing.
J Lab Clin Med. 1968 Sep;72(3):511-6 PMID: 4877134
The status of the antibiotic disc in Canada.
Can Med Assoc J. 1957 Feb 1;76(3):194-8 PMID: 13396673
Application of a microtechnique to viral serological investigations.
J Immunol. 1962 Mar;88:320-9 PMID: 13910995
Antibiotic susceptibility testing by a standardized single disk method.
Am J Clin Pathol. 1966 Apr;45(4):493-6 PMID: 5325707
The international situation with regard to the use of discs for antibiotic sensitivity tests.
Antibiot Annu. 1958-1959;6:833-5 PMID: 13637845
Single disc versus multiple disc and plate dilution techniques for antibiotic sensitivity testing.
Antibiot Annu. 1959-1960;7:574-80 PMID: 13797620
Evaluation of an automatic diluting device for microbiological applications.
Appl Microbiol. 1968 Sep;16(9):1414-6 PMID: 4970994
MICROMETHOD (SPOT-PLATE) DETERMINATION OF IN VITRO ANTIBIOTIC SUSCEPTIBILITY.
Appl Microbiol. 1965 Mar;13:279-80 PMID: 14325894

Article Info

Journal

Applied microbiology

Abbr.

Appl Microbiol

ISSN

0003-6919

DOI

10.1128/am.17.5.707-709.1969

Published

1969-05-00

Pages

707-9

Language

English

Region

United States

NLM ID

7605802

PMCID

PMC377784

Subset

External Links

PubMed source DOI: 10.1128/am.17.5.707-709.1969 PMC Full Text

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Tube dilution antimicrobial susceptibility testing: efficacy of a microtechnique applicable to diagnostic laboratories.

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