Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 63 ·No. 2 ·1969-06-00 ·Pages 384-91

Guthrie C, Nashimoto H, Nomura M

Abstract

E. coli mutants that fail to grow at 20 degrees C were isolated, and three such mutants were studied in detail. Two of these are defective in 50S ribosome biosynthesis at low temperatures and accumulate 43S and 32S particles, respectively. The third mutant produces reduced amounts of 50S ribosomes and accumulates 21S particles at low temperatures. The 43S and 32S particles contain 23S RNA and appear to be precursors of 50S ribosomes. The 21S particles contain 16S RNA. The mutation that causes accumulation of 32S particles is linked to the spc locus. Isolation and characterization of coldsensitive mutants should provide a fruitful approach to the study of in vivo ribosomal assembly.

MeSH Terms

Centrifugation, Density Gradient Chromosome Mapping Chromosomes, Bacterial Cold Temperature Escherichia coli/analysis Genetic Code Mutation Ribosomes/analysis Ultracentrifugation

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Guthrie C

Nashimoto H

Nomura M

References (7)

7 references, click to expand

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The synthesis and function of ribosomes in a new mutant of Escherichia coli.
Proc Natl Acad Sci U S A. 1967 Jan;57(1):141-7 PMID: 4860191
Structure and function of E. coli ribosomes. V. Reconstitution of functionally active 30S ribosomal particles from RNA and proteins.
Proc Natl Acad Sci U S A. 1968 Mar;59(3):777-84 PMID: 4868216
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Biosynthetic precursors of 30S and 50S ribosomal particles in Escherichia coli.
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.63.2.384

Published

1969-06-00

Pages

384-91

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC223576

Subset

External Links

PubMed source DOI: 10.1073/pnas.63.2.384 PMC Full Text View journal details

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Structure and function of E. coli ribosomes. 8. Cold-sensitive mutants defective in ribosome assembly.

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