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PMID: 4902811 Published · ppublish English Journal Article

Temperature-sensitive initiation of chromosome replication in a mutant of Escherichia coli.

Journal of bacteriology ·Vol. 100 ·No. 3 ·1969-12-00 ·Pages 1302-10

Kuempel PL

Abstract

The properties of Escherichia coli mutant D2-47LT indicate that it is temperature-sensitive for a protein required for the initiation of chromosome replication. The results of several different experiments are consistent with this hypothesis, and no support was found for the alternate hypotheses tested. Although the strain is usually unable to initiate replication at 42 C, some of the initiation proteins are apparently synthesized at the restrictive temperature. This can cause initiation on partially replicated, but not completed, chromosomes. It appears that the temperature-sensitive protein is required for initiation on completed chromosomes.

MeSH Terms
Bacterial Proteins/biosynthesis Carbon Isotopes Centrifugation, Density Gradient Chloramphenicol/pharmacology Chromosomes, Bacterial/growth & development DNA, Bacterial/biosynthesis Escherichia coli/drug effects,metabolism Mutation RNA, Bacterial/biosynthesis Temperature Thymine/metabolism
Chemicals
Bacterial Proteins Carbon Isotopes DNA, Bacterial RNA, Bacterial Chloramphenicol Thymine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Kuempel P L
References (24)
24 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.100.3.1302-1310.1969
Published
1969-12-00
Pages
1302-10
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC250320
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.100.3.1302-1310.1969 PMC Full Text View journal details
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