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PMID: 4905312 Published · ppublish English Journal Article

Interaction of ribosomes and the cell envelope of Escherichia coli mediated by lysozyme.

Journal of bacteriology ·Vol. 101 ·No. 2 ·1970-02-00 ·Pages 584-91

Patterson D, Weinstein M, Nixon R, Gillespie D

Abstract

Evidence is presented suggesting the existence of a natural ribosome-membrane complex. A reconstruction system is described wherein free ribosomes form a complex which appears to involve cell fragments. The reconstructed complex is similar in stability to the inferred natural complex. The reconstructed complex is generated by lysozyme, and it is concluded that at least part of the inferred natural complex is also generated by lysozyme. These results are discussed with reference to existing data concerning certain membrane-associated systems in bacteria.

MeSH Terms
Cell Membrane/enzymology Centrifugation, Density Gradient Chemical Precipitation Escherichia coli/enzymology,growth & development Muramidase/metabolism Protein Binding Ribosomes/enzymology Tritium Uridine/metabolism
Chemicals
Tritium Muramidase Uridine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Patterson D
Weinstein M
Nixon R
Gillespie D
References (22)
22 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.101.2.584-591.1970
Published
1970-02-00
Pages
584-91
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC284944
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.101.2.584-591.1970 PMC Full Text View journal details
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