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PMID: 4912521 Published · ppublish English Journal Article

Mutants of Escherichia coli K-12 with an altered glutamyl-transfer ribonucleic acid synthetase.

Journal of bacteriology ·Vol. 103 ·No. 1 ·1970-07-00 ·Pages 178-83

Murgola EJ, Adelberg EA

Abstract

Three streptomycin-suppressible lethal mutants of Escherichia coli K-12 have been shown to possess structurally altered glutamyl-transfer ribonucleic acid (tRNA) synthetases. Each mutant synthetase displays a K(m) value for glutamate which is 10-fold higher than the parental value, and the mutations reside in two widely separate loci on the genetic map. Mixing of the mutant extracts in pairs gave no indication of in vitro complementation. All three enzymes charge the minor tRNA(glu) fraction identically, but one (EM 120) charges the major fraction at a twofold lower rate than do the other two (EM 102 and EM 111). Possible explanations for the existence of the two synthetase loci are presented.

MeSH Terms
Carbon Isotopes Cellulose Chromosome Mapping Escherichia coli/enzymology,isolation & purification,metabolism Ethylamines Genetic Complementation Test Genetics, Microbial Glutamates/metabolism Hot Temperature Ligases/metabolism Mutation RNA, Transfer Streptomycin/metabolism
Chemicals
Carbon Isotopes Ethylamines Glutamates Cellulose RNA, Transfer Ligases Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murgola E J
Adelberg E A
References (34)
34 references, click to expand
  1. Histidine regulatory mutants in Salmonella typhimurium 3. A class of regulatory mutants deficient in tRNA for histidine.
    J Mol Biol. 1966 Dec 28;22(2):335-47 PMID: 5339689
  2. Isoleucine and valine metabolism of Escherichia coli. XV. Biochemical properties of mutants resistant to thiaisoleucine.
    J Bacteriol. 1968 May;95(5):1672-9 PMID: 4297021
  3. Multiple fractions of leucyl-transfer ribonucleic acid synthetase activity from Escherichia coli.
    Biochim Biophys Acta. 1966 Jul 20;123(1):134-41 PMID: 5336642
  4. [Thermosensitive mutations of systems activating valine in E. coli].
    Bull Soc Chim Biol (Paris). 1965;47(8):1609-26 PMID: 5321961
  5. Regulation of histidine biosynthetic enzymes in a mutant of Escherichia coli with an altered histidyl-tRNA synthetase.
    Mol Gen Genet. 1967;100(2):216-24 PMID: 4869993
  6. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES.
    J Bacteriol. 1965 Mar;89:706-11 PMID: 14273649
  7. Purification of five serine transfer ribonucleic acid species from Escherichia coli and their acylation by homologous and heterologous seryl transfer ribonucleic acid synthetases.
    J Biol Chem. 1970 Mar 25;245(6):1394-400 PMID: 4910052
  8. Studies on valyl-tRNA synthetase and tRNA from Escherichia coli. 3. Valyl-tRNA synthetases from thermosensitive mutants of Escherichia coli.
    J Mol Biol. 1969 Aug 28;44(1):31-45 PMID: 4897804
  9. EFFECT OF ALPHA-METHYLHISTIDINE ON THE CONTROL OF HISTIDINE SYNTHESIS.
    J Mol Biol. 1964 Sep;9:670-82 PMID: 14216610
  10. Tryptophanyl transfer ribonucleic acid synthetase from bovine pancreas. II. The chemically different subunits.
    J Biol Chem. 1969 Jul 25;244(14):3958-68 PMID: 5805407
  11. [On the properties of a modified alanyl-t-RNA synthetase in a strain of escherichia coli with thermosensible growth].
    C R Acad Sci Hebd Seances Acad Sci D. 1968 Jan 29;266(5):531-4 PMID: 4969238
  12. Revised linkage map of Escherichia coli.
    Bacteriol Rev. 1967 Dec;31(4):332-53 PMID: 4865540
  13. Prolyl transfer ribonucleic acid synthetase of Escherichia coli. I. Purification and evidence for subunits.
    J Biol Chem. 1969 Jan 25;244(2):223-30 PMID: 4886431
  14. Characterization of altered forms of glycyl transfer ribonucleic acid synthetase and the effects of such alterations on aminoacyl transfer ribonucleic acid synthesis in vivo.
    J Bacteriol. 1970 Apr;102(1):204-12 PMID: 4908672
  15. GLUTAMYL AND GLUTAMINYL RIBONUCLEIC ACID SYNTHETASES OF ESCHERICHIA COLI W. SEPARATION, PROPERTIES, AND STIMULATION OF ADENOSINE TRIPHOSPHATE-PYROPHOSPHATE EXCHANGE BY ACCEPTOR RIBONUCLEIC ACID.
    J Biol Chem. 1965 Jan;240:432-8 PMID: 14253448
  16. Phage-induced modification of valyl-tRNA synthetase.
    J Cell Physiol. 1969 Oct;74(2):Suppl 1:87+ PMID: 4902824
  17. Transfer RNA as a cofactor coupling amino acid synthesis with that of protein.
    Proc Natl Acad Sci U S A. 1968 Sep;61(1):229-36 PMID: 4972364
  18. Further investigations of the multiple fractions of leucyl transfer ribonucleic acid synthetase activity from Escherichia coli.
    Biochim Biophys Acta. 1970 Jan 21;199(1):79-85 PMID: 4905135
  19. Streptomycin-suppressible lethal mutations in Escherichia coli.
    J Bacteriol. 1970 Jul;103(1):20-6 PMID: 4912524
  20. DEMONSTRATION OF AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE IN A MUTANT OF ESCHERICHIA COLI.
    J Biol Chem. 1964 Jun;239:1839-43 PMID: 14213362
  21. Mutants of Escherichia coli with an altered tryptophanyl-transfer ribonucleic acid synthetase.
    J Bacteriol. 1968 Apr;95(4):1283-94 PMID: 4869215
  22. Genetic analysis of the glutamate permease in Escherichia coli K-12.
    J Bacteriol. 1969 Mar;97(3):1118-28 PMID: 4887500
  23. Mechanism of protein biosynthesis.
    Bacteriol Rev. 1969 Jun;33(2):264-301 PMID: 4896351
  24. Roles of amino acid activating enzymes in cellular physiology.
    Bacteriol Rev. 1966 Dec;30(4):701-19 PMID: 5342516
  25. PHENOTYPIC REPAIR BY STREPTOMYCIN OF DEFECTIVE GENOTYPES IN E. COLI.
    Proc Natl Acad Sci U S A. 1964 Mar;51:487-93 PMID: 14171463
  26. Mutants of Escherichia coli with an altered tyrosyl-transfer ribonucleic acid synthetase.
    J Bacteriol. 1969 Oct;100(1):167-75 PMID: 4898984
  27. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose.
    Biochemistry. 1967 Oct;6(10):3043-56 PMID: 6056973
  28. The participation of sRNA in the enzymatic synthesis of O-L-lysyl phosphatidylgylcerol in Staphylococcus aureus.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):934-41 PMID: 5219701
  29. Valyl-Transfer RNA: Role in Repression of the Isoleucine-Valine Enzymes in Escherichia coli.
    Science. 1967 Aug 18;157(3790):823-5 PMID: 17842790
  30. Methionyl transfer RNA synthetase mutants of Salmonella typhimurium which have normal control of the methionine biosynthetic enzymes.
    Biochim Biophys Acta. 1969 Jun 17;184(1):233-6 PMID: 4892972
  31. Histidine regulatory mutants in Salmonella typhimurium II. Histidine regulatory mutants having altered histidyl-tRNA synthetase.
    J Mol Biol. 1966 Dec 28;22(2):325-33 PMID: 5339688
  32. Subunit structure and function of Micrococcus cryophilus glutamyl transfer RNA synthetase.
    Biochim Biophys Acta. 1969 Oct 22;190(2):347-57 PMID: 5389134
  33. Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli. 3. Repression of enzymes of arginine biosynthesis in arginyl-tRNA synthetase mutants.
    J Mol Biol. 1968 Jul 14;35(1):83-93 PMID: 4939781
  34. Renaturation of transfer ribonucleic acids through site binding of magnesium.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):941-8 PMID: 5327073
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.103.1.178-183.1970
Published
1970-07-00
Pages
178-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC248054
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.103.1.178-183.1970 PMC Full Text View journal details
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