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PMID: 4914074 Published · ppublish English Journal Article

Mechanism of action of nalidixic acid on conjugating bacteria.

Journal of bacteriology ·Vol. 102 ·No. 3 ·1970-06-00 ·Pages 688-701

Bouck N, Adelberg EA

Abstract

When nalidixic acid, a specific and effective inhibitor of cellular deoxyribonucleic acid synthesis, is added to conjugating bacteria at any time during mating, it stops genetic transfer provided the donor bacterium is sensitive to the drug. When this inhibition is released by the removal of the nalidixic acid, transfer does not resume at the point on the chromosome where it was stopped, but begins again at the transfer origin. Curves relating the effects of various low doses of nalidixic acid to the frequency of recombination reveal that several "hits" are necessary to inhibit recombination for early markers. The number of required "hits" decreases as the distance of the marker from the transfer origin increases. Transfer between drug-resistant cells may also be inhibited by nalidixic acid. The effect of high drug doses on matings between resistant cells is similar to that of low drug doses on matings with a sensitive male.

MeSH Terms
Agar Carbon Isotopes Chromosome Mapping Conjugation, Genetic Crosses, Genetic DNA, Bacterial/biosynthesis Drug Resistance, Microbial Escherichia coli/drug effects,growth & development,metabolism Nalidixic Acid/pharmacology Recombination, Genetic Thymidine/metabolism
Chemicals
Carbon Isotopes DNA, Bacterial Nalidixic Acid Agar Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Bouck N
Adelberg E A
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39 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.102.3.688-701.1970
Published
1970-06-00
Pages
688-701
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC247614
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.102.3.688-701.1970 PMC Full Text View journal details
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