Cytoplasmic particles and aminoacyl transferase I activity.

The Journal of cell biology ·Vol. 45 ·No. 1 ·1970-04-00 ·Pages 1-8

Shelton E, Kuff EL, Maxwell ES, Harrington JT

Abstract

It has been possible to show by electron microscopy of samples selected from sucrose gradients that particles of specific size and shape are present in supernatant fluids derived from nucleated animal and plant cells, but not in extracts from Escherichia coli. Aminoacyl transferase I activity in these same gradients sediments in two peaks representing material of approximately 5-7S and 18-20S. A rectangular particle, 100 x 145 A in size, sediments at 19S and coincides with the second peak of transferase I activity. The possibility that the rectangular particle may be a "carrier" particle associated with transferase I is discussed.

MeSH Terms

Animals Centrifugation, Density Gradient Cytoplasmic Granules/enzymology Escherichia coli/enzymology Humans Liver/enzymology Microscopy, Electron Plants, Edible/enzymology Plasmacytoma/enzymology Reticulocytes/enzymology Ribosomes Transferases/metabolism

Chemicals

Transferases

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Shelton E

Kuff E L

Maxwell E S

Harrington J T

References (9)

9 references, click to expand

The binding of aminoacyl transferase II to ribosomes.
Biochem Biophys Res Commun. 1967 Jun 9;27(5):568-72 PMID: 6033141
Ultrastructure of gamma-M immunoglobulin and alpha macroglobulin: electron-microscopic study.
Science. 1967 Nov 17;158(3803):933-6 PMID: 4167979
Amino acid transfer factors from yeast. Isolation and properties of three different transfer-active protein fractions.
Biochemistry. 1967 Nov;6(11):3569-75 PMID: 6073038
Release of a macromolecular protein component from human erythrocyte ghosts.
Biochim Biophys Acta. 1968 Apr 29;150(3):534-7 PMID: 5650402
Evidence for aminoacyl-tRNA binding, peptide bond synthesis, and translocase activities in the aminoacyl transfer reaction.
Arch Biochem Biophys. 1968 May;125(2):497-505 PMID: 5656804
The isolation and biological activity of multiple forms of aminoacyl transferase I of rat liver.
Biochim Biophys Acta. 1968 Aug 23;166(1):58-67 PMID: 5698066
Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome.
Nature. 1963 Feb 2;197:430-5 PMID: 14000153
ON THE CYTOLOGICAL UNIT FOR PROTEIN SYNTHESIS IN VIVO IN E. COLI. I. STUDIES WITH SPHEROPLASTS OF TYPE K-12.
Biochim Biophys Acta. 1964 Feb 17;80:279-93 PMID: 14164210
RESOLUTION OF AMINOACYL-TRANSFERRING ENZYMES FROM RAT LIVER BY MOLECULAR SIEVE CHROMATOGRAPHY.
J Biol Chem. 1965 Aug;240:3346-52 PMID: 14321371

Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.45.1.1

Published

1970-04-00

Pages

1-8

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2107991

Subset

External Links

PubMed source DOI: 10.1083/jcb.45.1.1 PMC Full Text View journal details

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