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PMID: 4920093 Published · ppublish English Journal Article

In vitro transcription of a transfer RNA gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 4 ·1970-08-00 ·Pages 1260-6

Daniel V, Sarid S, Beckmann JS, Littauer UZ

Abstract

The Escherichia coli tRNA(Tyr) gene carried by the varphi80psu(3) + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNA(Tyr)-like polynucleotide chains were detected by competition with purified E. coli(32)P-tRNA(Tyr) for specific hybridization sites on varphi80psu(3) + DNA. Analysis by sucrose gradient centrifugation showed the tRNA(Tyr)-like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor rho during the transcription did not significantly reduce the average size of the in vitro synthesized tRNA(Tyr)-like chains.

MeSH Terms
Bacteriophages/metabolism Carbon Isotopes Centrifugation, Density Gradient Chromatography, DEAE-Cellulose DNA/isolation & purification Escherichia coli/enzymology,metabolism Genetic Code Hybridization, Genetic In Vitro Techniques Phosphorus Isotopes RNA/biosynthesis RNA Nucleotidyltransferases/metabolism RNA, Transfer/metabolism Tritium
Chemicals
Carbon Isotopes Phosphorus Isotopes Tritium RNA DNA RNA, Transfer RNA Nucleotidyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Daniel V
Sarid S
Beckmann J S
Littauer U Z
References (23)
23 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.66.4.1260
Published
1970-08-00
Pages
1260-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335814
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.66.4.1260 PMC Full Text View journal details
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