Proteolytic reaction of mammalian spermatozoa on gelatin membranes.

Science (New York, N.Y.) ·Vol. 170 ·No. 3959 ·1970-11-13 ·Pages 749-51

Gaddum P, Blandau RJ

Abstract

The acrosomes of spermatozoa of several mammalian species show proteolytic activity when applied to fixed gelatin membranes. The technique permits continuous observation of the enzymatic reaction of an individual spermatozoon. Release of the enzyme occurs solely in the region of the acrosome, in a manner which is species-specific.

MeSH Terms

Animals Cattle Cricetinae Gelatin/metabolism Guinea Pigs Humans Male Membranes Mice Microscopy, Phase-Contrast Peptide Hydrolases/metabolism Rabbits Rats Species Specificity Spermatozoa/enzymology

Chemicals

Gelatin Peptide Hydrolases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gaddum P

Blandau R J

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.170.3959.749

Published

1970-11-13

Pages

749-51

Language

English

Region

United States

NLM ID

0404511

Subset

External Links

PubMed source DOI: 10.1126/science.170.3959.749 View journal details

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Proteolytic reaction of mammalian spermatozoa on gelatin membranes.

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