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PMID: 4922285 Published · ppublish English Journal Article

Breakdown by streptomycin of initiation complexes formed on ribosomes of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 3 ·1970-11-00 ·Pages 1148-55

Modolell J, Davis BD

Abstract

Streptomycin induces breakdown of the completed 70S initiation complex on ribosomes of Escherichia coli, but it does not interfere with any step in the formation of the complex. Moreover, it does not appear to interact with the ribosome in any special way during initiation, since the kinetics of breakdown are the same whether streptomycin is added before formation of the initiation complex, or after its completion, or (as previously observed) after formation of a polypeptide. fMet-tRNA is released as such, without chain elongation; it is released from a puromycin-reactive ("P") site. Streptomycin thus appears to distort not only the A site of the ribosome (as suggested earlier) but also the P site.

MeSH Terms
Bacterial Proteins/biosynthesis Binding Sites Escherichia coli/drug effects Genetic Code Ribosomes/drug effects Streptomycin/pharmacology
Chemicals
Bacterial Proteins Streptomycin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Modolell J
Davis B D
References (22)
22 references, click to expand
  1. Rapid inhibition of polypeptide chain extension by streptomycin.
    Proc Natl Acad Sci U S A. 1968 Dec;61(4):1279-86 PMID: 4884680
  2. Antibiotic inhibitors of the bacterial ribosome.
    Bacteriol Rev. 1968 Dec;32(4 Pt 2):493-528 PMID: 4179192
  3. Role of guanosine 5'-triphosphate in the initiation of Peptide synthesis, I. Synthesis of formylmethionyl-puromycin.
    Proc Natl Acad Sci U S A. 1967 Mar;57(3):759-66 PMID: 16591528
  4. Binding of formylmethionyl-tRNA to 30S ribosomal sub-units.
    Nature. 1968 Mar 16;217(5133):1013-6 PMID: 4868440
  5. Studies on polynucleotides, LXXXIV. On the role of ribosomal subunits in protein synthesis.
    Proc Natl Acad Sci U S A. 1967 Dec;58(6):2455-61 PMID: 4873591
  6. Studies of ribosomal subunit exchange.
    Cold Spring Harb Symp Quant Biol. 1969;34:209-20 PMID: 4909500
  7. The role of guanosine 5'-triphosphate in the initiation of peptide synthesis. 3. Binding of formylmethionyl-tRNA to ribosomes.
    Proc Natl Acad Sci U S A. 1967 Oct;58(4):1638-44 PMID: 4867668
  8. Effect of spectinomycin on polypeptide synthesis in extracts of Escherichia coli.
    J Mol Biol. 1967 Oct 14;29(1):203-15 PMID: 4228335
  9. The ribosome dissociation factor and the ribosome-polysome cycle.
    Cold Spring Harb Symp Quant Biol. 1969;34:223-30 PMID: 4985887
  10. Enzymatic hydrolysis of peptidyl-tRNA.
    Biochem Biophys Res Commun. 1968 Apr 5;31(1):37-42 PMID: 4869944
  11. Enzymatic hydrolysis of N-substituted aminoacyl-tRNA.
    Proc Natl Acad Sci U S A. 1967 Nov;58(5):2079-86 PMID: 4866985
  12. RNA CODEWORDS AND PROTEIN SYNTHESIS. THE EFFECT OF TRINUCLEOTIDES UPON THE BINDING OF SRNA TO RIBOSOMES.
    Science. 1964 Sep 25;145(3639):1399-407 PMID: 14172630
  13. The contrasting role of strA and ram gene products in ribosomal functioning.
    Cold Spring Harb Symp Quant Biol. 1969;34:101-9 PMID: 4909486
  14. On the specificity and stability of an enzyme that hydrolyzes N-substituted aminoacyl-transfer RNA's.
    Proc Natl Acad Sci U S A. 1968 Oct;61(2):701-7 PMID: 4879402
  15. Translation and translocation of defined RNA messengers.
    J Mol Biol. 1969 Feb 14;39(3):441-60 PMID: 4902905
  16. Junction of the 50S ribosomal subunit with the 30S initiation complex.
    Nature. 1968 Oct 19;220(5164):244-7 PMID: 5684849
  17. A GTP requirement for binding initiator tRNA to ribosomes.
    Nature. 1967 Jul 29;215(5100):490-2 PMID: 4862225
  18. Polyribosome metabolism in Escherichia coli treated with chloramphenicol, neomycin, spectinomycin or tetracycline.
    J Mol Biol. 1969 Oct 28;45(2):205-20 PMID: 4243913
  19. Ribosomes from Escherichia coli merodiplods heterozygous for resistance to streptomycin and to spectinomycin.
    J Mol Biol. 1968 Nov 14;37(3):407-21 PMID: 4238086
  20. Mechanism of inhibition of ribosomes by streptomycin.
    Nature. 1969 Oct 25;224(5217):345-8 PMID: 5343879
  21. Mechanism of action of streptomycin in E. coli: interruption of the ribosome cycle at the initiation of protein synthesis.
    Proc Natl Acad Sci U S A. 1968 Jul;60(3):873-80 PMID: 4875806
  22. Purification and properties of initiation factor f-1.
    Nature. 1969 Jun 7;222(5197):944-7 PMID: 5789323
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.3.1148
Published
1970-11-00
Pages
1148-55
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283330
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.3.1148 PMC Full Text View journal details
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