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PMID: 4922291 Published · ppublish English Journal Article

Identification of the cytokinin-active ribonucleosides in pure Escherichia coli tRNA species.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 3 ·1970-11-00 ·Pages 1448-53

Bartz J, Söll D, Burrows WJ, Skoog F

Abstract

The distribution of the two cytokinin-active ribonucleosides 6-(3-methyl-2-butenylamino)-9-beta-D-ribofuranosylpurine (1) and 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine (2) in tRNA(Phe), tRNA(UUG) (Leu), tRNA(UCR) (Ser), tRNA(Tyr), tRNA(Cys), and tRNA(2) (Trp) from stationary phase Escherichia coli has been studied. Compound 2 predominated in all these species except tRNA(Phe), in which compound 1 was greatly in excess. Compound 1 was present in relatively small amounts in tRNA(UUG) (Leu), tRNA(Cys), and tRNA(2) (Trp), and was not detected in tRNA(UCR) (Ser) and tRNA(Tyr). Cells from the early logarithmic phase had the highest cytokinin content in their tRNA. Stationary phase tRNA had a slight increase in the amount of 1, which may be explained by the appearance of the new tRNA(Phe). No differences were observed between the chromatographic patterns of tRNAs from early-, mid-, and latelogarithmic phases.

MeSH Terms
Amino Acids/analysis Chromatography Escherichia coli/growth & development Kinins/isolation & purification Methods Nucleosides/isolation & purification RNA Nucleotidyltransferases RNA, Bacterial/analysis RNA, Transfer/analysis
Chemicals
Amino Acids Kinins Nucleosides RNA, Bacterial RNA, Transfer RNA Nucleotidyltransferases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bartz J
Söll D
Burrows W J
Skoog F
References (18)
18 references, click to expand
  1. Cytokinin activity: localization in transfer RNA preparations.
    Science. 1966 Dec 9;154(3754):1354-6 PMID: 5332571
  2. Purification of methionine-, valine-, phenylalanine- and tyrosine-specific tRNA from Escherichia coli.
    Biochim Biophys Acta. 1967 Jun 20;142(1):133-48 PMID: 4860476
  3. Studies on polynucleotides. LXXV. Specificity of tRNA for codon recognition as studied by the ribosomal binding technique.
    J Mol Biol. 1967 Oct 14;29(1):97-112 PMID: 4861614
  4. Cytokinin from soluble RNA of Escherichia coli: 6-(3-methyl-2-butenylamino)-2-methylthio-9-beta-D-ribofuranosylpurine.
    Science. 1968 Aug 16;161(3842):691-3 PMID: 4874577
  5. Transfer ribonucleic acids in Escherichia coli. Multiplicity and variation.
    Biochemistry. 1968 Aug;7(8):2799-808 PMID: 4299084
  6. A general procedure for the isolation of specific transfer ribonucleic acids.
    Biochemistry. 1968 Oct;7(10):3459-68 PMID: 5681458
  7. The presence of 2-methylthio-N6-(delta-2-isopentenyl) adenosine in serine and phenylalanine transfer RNA's from Escherichia coli.
    Biochim Biophys Acta. 1969 Apr 22;179(2):517-20 PMID: 4890605
  8. Mechanism of protein biosynthesis.
    Bacteriol Rev. 1969 Jun;33(2):264-301 PMID: 4896351
  9. The isolation and identification of two cytokinins from Escherichia coli transfer ribonucleic acids.
    Biochemistry. 1969 Jul;8(7):3071-6 PMID: 4897210
  10. Cytokinins: distribution in transfer RNA species of Escherichia coli.
    Proc Natl Acad Sci U S A. 1969 Jul;63(3):834-41 PMID: 4899879
  11. Isolation of cytokinins from tRNA.
    Biochem Biophys Res Commun. 1969 Oct 22;37(3):451-6 PMID: 4900138
  12. Two species of Bacillus subtilis tyrosine transfer ribonucleic acid. Biological properties and alteration in their relative amounts during growth.
    J Biol Chem. 1969 Nov 10;244(21):5959-66 PMID: 4981787
  13. An iron-dependent modification of several transfer RNA species in Escherichia coli.
    J Mol Biol. 1969 Dec 28;46(3):581-4 PMID: 4904109
  14. Purification of five serine transfer ribonucleic acid species from Escherichia coli and their acylation by homologous and heterologous seryl transfer ribonucleic acid synthetases.
    J Biol Chem. 1970 Mar 25;245(6):1394-400 PMID: 4910052
  15. The nucleotide sequences of tyrosine transfer RNAs of Escherichia coli.
    Eur J Biochem. 1970 Apr;13(3):461-83 PMID: 4315419
  16. Levels of 5,6-dihydrouridine in relaxed and chloramphenicol transfer ribonucleic acid.
    Biochemistry. 1970 Jun 9;9(12):2513-9 PMID: 4912486
  17. Role modifications in tyrosine transfer RNA: a modified base affecting ribosome binding.
    J Mol Biol. 1969 Jan 14;39(1):145-57 PMID: 4938812
  18. Physiologically induced changes in the property of phenylalanine tRNA in Escherichia coli.
    J Mol Biol. 1968 Nov 28;38(1):25-40 PMID: 4941472
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.67.3.1448
Published
1970-11-00
Pages
1448-53
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC283372
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.67.3.1448 PMC Full Text View journal details
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