DNA ligase mutants of Escherichia coli.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 67 ·No. 3 ·1970-11-00 ·Pages 1580-7

Gellert M, Bullock ML

Abstract

A procedure is described for the isolation of Escherichia coli mutants with either excess or deficient DNA ligase activity. A mutant that overproduces DNA ligase supports the growth of ligase-defective (gene 30 mutant) T4 phages. Even T4 rII-gene 30 double mutants, which are able to grow in normal E. coli, cannot grow in cells deficient in DNA ligase. A functional DNA ligase, supplied either by the phage or the host, thus seems to be required for T4 growth. An E. coli strain that makes a temperature-sensitive DNA ligase becomes radiation-sensitive at high temperature, but otherwise grows normally and shows no obvious defect in DNA replication.

MeSH Terms

Centrifugation, Density Gradient DNA, Bacterial Escherichia coli/enzymology Genetics, Microbial Ligases/metabolism Mutation Temperature

Chemicals

DNA, Bacterial Ligases

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gellert M

Bullock M L

References (27)

27 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.67.3.1580

Published

1970-11-00

Pages

1580-7

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283393

Subset

External Links

PubMed source DOI: 10.1073/pnas.67.3.1580 PMC Full Text View journal details

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DNA ligase mutants of Escherichia coli.

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